| 多花黑麦草原生质体培养和再生白化苗 |
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| 引用本文： | 陈文品,吴琴生,刘大钧.多花黑麦草原生质体培养和再生白化苗[J].南京农业大学学报,1989(4). |
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| 作者姓名： | 陈文品 吴琴生 刘大钧 |
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| 作者单位： | 南京农业大学遗传育种教研组
(陈文品，吴琴生)，南京农业大学遗传育种教研组(刘大钧) |
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| 摘 要： | 本文报道从多花黑麦单(Lolium multiflorum Lam.)原生质体培养获得愈伤组织并再生白化苗的试验结果。利用多花黑麦草幼穗在MS 3mg/L2.4-D的培养基上诱导出愈伤组织,并在MS 2mg/L2.4-D的液体培养基中振荡培养,产生悬浮细胞系。从悬浮细胞制备原生质体,用看护培养,琼脂糖固体平板培养和液体浅层培养均获得了再生细胞团。这些细胞团在MS 1mg/L2.4-D 60g/L蔗糖的培养基上形成愈伤组织和胚状体,并在分化培养基上再生出白化苗。试验还对有关培养基进行了研究。
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| 关 键 词： | 多花黑麦草 原生质体 愈伤组织 胚状体 白化苗 |
PROTOPLAST CULTURE AND ALBINO-PLANTLET FORMATION OF LOLIUM MULTIFLORUM LAM. |
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| Chen Wenpin,Wu Qinsheng and Liu Dajun.PROTOPLAST CULTURE AND ALBINO-PLANTLET FORMATION OF LOLIUM MULTIFLORUM LAM.[J].Journal of Nanjing Agricultural University,1989(4). |
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| Authors: | Chen Wenpin Wu Qinsheng and Liu Dajun |
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| Abstract: | Protoplasts isolated from suspension cells of annual ryegrass (Lolium multiflorum Lam.cv.Multimo) were used to investigate callus formation and plantlet differentiation. The suspension culture was initiated in MS 2mg/L 2,4-D medium from young inflorescence calli which were cultured in MS 3mg/L 2.4-D medium. The regenerated protoelones were obtained through mixed nurse, agarose plate and liquid shallow layer culture methods. The calli and embryoids were formed from the protoelones in MS 1mg/L 2.4-D 60g/L, sucrose medium. The albino-plantlets have been differentiated from the embryoids in regeneration medium. Several protoplast culture media were used and studied in the experiments. |
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| Keywords: | annual ryegrass protoplast callus embryoid albinoplantlet |
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