| 南瓜热激蛋白相关基因CmHSP70-5 的克隆和高温胁迫下的表达分析 |
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| 引用本文： | 胡艳平,周扬,杨春,廖道龙,朱白婢,王敏,黄文枫,张文,云天海.南瓜热激蛋白相关基因CmHSP70-5 的克隆和高温胁迫下的表达分析[J].中国蔬菜,2019,1(8):28-33. |
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| 作者姓名： | 胡艳平 周扬 杨春 廖道龙 朱白婢 王敏 黄文枫 张文 云天海 |
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| 作者单位： | 1. 海南省农业科学院蔬菜研究所，海南省蔬菜生物学重点实验室，海南省瓜菜育种工程技术研究中心，;
海南海口571100；;2.海南大学园艺学院，海南海口570228；;3. 海南省农业科学院，海南海口571100 |
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| 基金项目： | 海南省自然科学基金面上项目（20163084），海南省瓜菜育
种工程技术研究中心开放课题（GCYZGCZX2017001） |
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| 摘 要： | 基于南瓜基因组序列，采用PCR 的方法从南瓜cDNA 中克隆1 个热激蛋白相关基因，命名为CmHSP70-5，采
用生物信息学方法分析该基因编码的蛋白与其同源蛋白的系统发育关系，利用荧光定量PCR 研究南瓜幼苗在高温胁迫下
CmHSP70-5 基因的表达量。结果表明：CmHSP70-5 基因全长1 953 bp，其编码的CmHSP70-5 蛋白与拟南芥AtHSP70-5
的亲缘关系最近。未经高温处理时CmHSP70-5 基因在根中的表达量要显著高于茎和叶。在高温胁迫下，根、茎、叶中
CmHSP70-5 基因均呈现上调表达，均在处理后3 h 表达量达到最大，说明CmHSP70-5 基因的表达与南瓜高温胁迫有关。
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| 关 键 词： | 南瓜 高温胁迫 CmHSP70-5 基因 基因表达 |
Cloning of Heat Shock Protein Related Gene CmHSP70-5 and Expression Analysis under Heat Stress in Cucurbita moschata |
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| HU Yan-ping,ZHOU Yang,YANG Chun,LIAO Dao-long,ZHU Bai-bi,WANG Min,HUANG Wen-feng,ZHANG Wen,YUN Tian-hai.Cloning of Heat Shock Protein Related Gene CmHSP70-5 and Expression Analysis under Heat Stress in Cucurbita moschata[J].China Vegetables,2019,1(8):28-33. |
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| Authors: | HU Yan-ping ZHOU Yang YANG Chun LIAO Dao-long ZHU Bai-bi WANG Min HUANG Wen-feng ZHANG Wen YUN Tian-hai |
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| Institution: | 1. Institute of Vegetables，Hainan Academy of Agricultural Sciences，Hainan Key Laboratory of Vegetable Biology，Hainan;
Vegetable Breeding Engineering Technology Research Center，Haikou 571100，Hainan，China；;2. College of Horticulture，;
Hainan University，Haikou 570228，Hainan，China；;3.Hainan Academy of Agricultural Sciences，Haikou 571100，Hainan，;
China |
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| Abstract: | A heat shock protein gene，naming CmHSP70-5，was isolated from pumpkin cDNA by
polymerase chain reaction （PCR） method based on pumpkin genome sequence．Phylogenetic relationship
of HSP70-5 and its homology were analyzed using bioinformation method．The expression quantity of
CmHSP70-5 under heat stress was studied by real-time PCR．The results showed that CmHSP70-5 gene was 1
953 bp，and its coding CmHSP70-5 protein had the closest relative with Arabidopsis AtHSP70-5．CmHSP70-5
gene had a high expression level in roots than in stems and leaves under normal conditions．Real-time PCR
results indicated that under heat stress，the expression of CmHSP70-5 gene in roots，stems and leaves were all
up-regulated and could reach the maximum quantity 3 hours after treatment，indicating that the expression of
CmHSP70-5 gene was related to heat stress in Cucurbita moschata． |
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| Keywords: | Cucurbita moschata Heat stress CmHSP70-5 gene Gene expression |
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