| 基于InDel标记的大白菜育种材料分子身份证构建 |
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| 引用本文： | 刘栓桃,张志刚,王荣花,王立华,李巧云,赵智中.基于InDel标记的大白菜育种材料分子身份证构建[J].中国蔬菜,2019,1(2):34-41. |
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| 作者姓名： | 刘栓桃 张志刚 王荣花 王立华 李巧云 赵智中 |
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| 作者单位： | （山东省农业科学院蔬菜花卉研究所，国家蔬菜改良中心山东分中心，山东省设施蔬菜生物学重点实验室，;
山东济南 250100） |
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| 基金项目： | 山东省重点研发计划项目(2017GNC10124);山东省农业重大应用技术创新项目(2018);山东省泰山学者计划项目(2016-2020);山东省农业科学院创新工程项目(CXGC2016A11) |
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| 摘 要： | 以105份大白菜育种材料为试材,精选20对均匀分布于大白菜基因组、且只能检测出2个等位基因的共显性InDel标记引物进行分子标记检测,构建分子身份证。结果表明:20对引物中有16对引物在所有材料中均扩增出单一条带,另外各有2对引物在部分材料中存在缺失和加倍现象。将所有标记引物按其所处染色体编号由小(A01)到大(A10)排列,同一染色体上的标记则按照物理位置从小到大排列,按顺序对标记赋值,构建了105份大白菜材料的分子身份证。所筛选的20对引物对未知新种质的身份证构建具有通用性和兼容性,表明InDel标记技术可以作为大白菜鉴定和分子身份证构建的有效技术手段。
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| 关 键 词： | 大白菜 育种材料 INDEL标记 分子身份证 |
Construction of Molecular Identity Card for Chinese Cabbage Breeding Materials Based on InDel Markers |
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| LIU Shuan-tao,ZHANG Zhi-gang,WANG Rong-hua,WANG Li-hua,LI Qiao-yun,ZHAO Zhi-zhong.Construction of Molecular Identity Card for Chinese Cabbage Breeding Materials Based on InDel Markers[J].China Vegetables,2019,1(2):34-41. |
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| Authors: | LIU Shuan-tao ZHANG Zhi-gang WANG Rong-hua WANG Li-hua LI Qiao-yun ZHAO Zhi-zhong |
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| Institution: | (Vegetable and Flower Institute of Shandong Academy of Agricultural Sciences,Shandong Branch of National Center forVegetables Improvement,Shandong Key Laboratory for Biology of Greenhouse Vegetables,Ji’nan 250100,Shandong,China) |
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| Abstract: | Taking 105 Chinese cabbage Brassica campestris L. ssp. pekinensis (Lour) Olsson] breeding lines as test material,this paper screened out 20 pairs distributed evenly in Chinese cabbage genome,conducted molecular marker detection on the codominant InDel primers only produced 2 alleles,and built molecular identity cards.The results showed that 16 out of 20 pairs of primers produced single band,respectively. While,2 pairs of primers each from partial materials were found loss and duplicated.All primers were ordered according to chromosome number,starting from A01 to A10 and primers on the same chromosome were ordered according to their physical position from small to large.According to these order,values were assigned to tags and 105 molecular identity cards of Chinese cabbage material were built.The screened out 20 pairs of primers had universality and compatibility for the identity cards construction of the unknown new germplasm resources,indicating that InDel marker technology could be used as an effective means for Chinese cabbage identification and molecular ID construction. |
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| Keywords: | Chinese cabbage Breeding line InDel marker Molecular ID(identity card) |
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