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| 黄瓜抗感材料接种尖孢镰孢菌后根系游离氨基酸的差异分析 | |
| 引用本文： | 李亚莉,侯 栋,岳宏忠,张东琴.黄瓜抗感材料接种尖孢镰孢菌后根系游离氨基酸的差异分析[J].中国蔬菜,2019,1(9):44-49. |
| 作者姓名： | 李亚莉 侯 栋 岳宏忠 张东琴 |
| 作者单位： | （甘肃省农业科学院蔬菜研究所，甘肃兰州730070） |
| 基金项目： | 甘肃省农业科学院中青年基金项目（2016GAAS45），国家 大宗蔬菜产业技术体系兰州综合试验站项目（CARS-23-G-19），甘 肃省科技重大专项瓜菜新品种选育项目（17ZD2NA015-04） |
| 摘 要： | 为阐明尖孢镰孢菌侵染黄瓜抗感材料后根系游离氨基酸的差异，对黄瓜抗感材料进行接种，以接种灭菌水为对照，分析接种后8、12、16、20 d 根系游离氨基酸的动态变化。结果表明：接种病原菌后，抗病材料游离氨基酸总量呈下降趋势，感病材料游离氨基酸总量呈上升趋势。抗感材料接种病原菌处理的游离氨基酸总量均较对照增加，接种后8、12、16、20 d，抗病材料分别较对照增加9.37%、38.81%、33.09%、25.72%，感病材料分别较对照增加19.89%、49.49%、101.11%、208.96%，游离氨基酸总量受病原菌的影响表现为感病材料大于抗病材料。接种病原菌后，β- 丙氨酸、鸟氨酸、亮氨酸、天冬氨酸、苯丙氨酸、赖氨酸、尿素、精氨酸和γ- 氨基丁酸在抗感材料中的变化规律明显不同。 |
| 关 键 词： | 黄瓜 抗感材料 尖孢镰孢菌 根系 游离氨基酸 |
Difference Analysis on Root System Free Amino Acid after Inoculating Fusarium oxysporum to Resistant and Susceptible Cucumber Materials |
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| LI Ya-li,HOU Dong,YUE Hong-zhong,ZHANG Dong-qin.Difference Analysis on Root System Free Amino Acid after Inoculating Fusarium oxysporum to Resistant and Susceptible Cucumber Materials[J].China Vegetables,2019,1(9):44-49. | |
| Authors: | LI Ya-li HOU Dong YUE Hong-zhong ZHANG Dong-qin |
| Institution: | （Vegetable Institute，Gansu Academy of Agricultural Sciences，Lanzhou 730070，Gansu，China） |
| Abstract: | To clarify differences in root free amino acid contents after inoculating the resistant and susceptible cucumbers with Fusarium oxysporum，this paper conducted inoculation on these cucumber materials taking sterile water as the contrast．8，12，16，20 days after inoculation，the dynamic changes in root system free amino acid content were analyzed．The results showed that the total free amino acid content of resistant material was on a declining trend，after Fusarium oxysporum inoculation．While， that in susceptible material maintained an upward trend．The total free amino acid contents of resistant and susceptible materials treated with Fusarium oxysporum inoculation were all increased than that of the contrast． 8，12，16 and 20 days after inoculation，the total free amino acid contents in resistant material increased by 9.37%，38.81%，33.09% and 25.72%，respectively．That in susceptible material increased by 19.89%， 49.49%，101.11% and 208.96%，respectively．The total free amino acid contents after Fusarium oxysporum inoculation in susceptible ＞ resistant material．After inoculation，the changing rules of β–alanie，Ornithine， Leucine，Aspartic acid，Phenylalanine，Lysine，Urea，Arginine and γ- aminobutyric contents in resistant and susceptible materials were obviously different． |
| Keywords: | Cucumber Resistant and susceptible materials Fusarium oxysporum Root system Free amino acid |
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