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| 辣椒疫霉病生防细菌的筛选鉴定及其防效 | |
| 引用本文： | 赵旖森,张亮,盛浩,袁红,周清.辣椒疫霉病生防细菌的筛选鉴定及其防效[J].中国蔬菜,2019,1(1):65-69. |
| 作者姓名： | 赵旖森 张亮 盛浩 袁红 周清 |
| 作者单位： | 湖南农业大学资源环境学院，湖南长沙 410128 |
| 基金项目： | 国家自然科学基金项目（41571234），湖南农业大学“1515” 学术创新团队带头人培养项目，湖南农业大学青年科学基金项目，湖 南农业大学大学生科技创新项目（2017ZK36） |
| 摘 要： | 以筛选、获取优良辣椒疫霉病害生防菌株材料为目标，通过平板稀释法与对峙法对水稻土壤耕层可培养细菌进行
分离和筛选，成功从167 株可培养细菌中分离、筛选获得1 株拮抗菌LRS-1，并被鉴定为多粘类芽孢杆菌（Paenibacillus
polymyxa）。该拮抗菌对辣椒疫霉菌具有68% 左右的室内持久抑菌率以及63.4% 的温室病害防效；Realtime-PCR 检测结果表
明该拮抗菌对根围疫霉菌侵染数量具有显著的抑制效果。 |
| 关 键 词： | 辣椒疫霉 拮抗菌 筛选 鉴定 防效 |
Screening，Identification and Control Effect of a Bio-control Bacteria for Pepper Phytophthora Blight |
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| ZHAO Yi-sen,ZHANG Liang,SHENG Hao,YUAN Hong,ZHOU Qing.Screening，Identification and Control Effect of a Bio-control Bacteria for Pepper Phytophthora Blight[J].China Vegetables,2019,1(1):65-69. | |
| Authors: | ZHAO Yi-sen ZHANG Liang SHENG Hao YUAN Hong ZHOU Qing |
| Institution: | College of Resource and Environment，Hunan Agricultural University，Changsha 410128，Hunan，China |
| Abstract: | In order to screen and obtain good bio-control bacterial strain，this study isolated and screened cultivable bacteria from the top paddy soil by means of dilution method and inhibition test in plate. An effective antagonistic bacteria named LRS-1 was isolated from 167 cultivable bacteria strains. It was identified as Paenibacillus polymyxa bacteria. The strain owned as much as 68% inhibition rate against Phytophthora capsici in door and 63.4% controlling effect on Phytophthora blight of pepper in greenhouse. The result of Real-time PCR test indicated that this strain had remarkable inhibitory effect on Phytophthora pathogen population in rhizosphere. |
| Keywords: | Phytophthora capsici Antagonistic bacteria Screen Identification Control effect |
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