Changes in proline-14C metabolism in barley seedlings germinating at low temperature |
| |
| Authors: | Nobuyuki Shiomi Shiro Hori |
| |
| Institution: | Department of Applied Biology , Radiation Center of Osaka Prefecture , Sakai , Osaka , Japan |
| |
| Abstract: | Changes in the metabolic pattern of proline-14C were examined in barley seedlings germinated at the low temperature of 2°C (LT) and compared with those germinated at 25°C (HT). In the LT shoots. proline-14C incorporation was higher in the cationic fraction and lower in the acid-neutral fraction than that of HT, respectively. More proline-14C of LT was converted to other amino acids, especially to acidic amino acids in free amino acid state, than was the HT proline-14C which had a comparatively wide distribution. In the LT protein fractions. more proline-14C was incorporated into the cytoplasmic protein than into the cell wall protein. On the contrary, the radioactivity of the lIT cell wall increased more distinctively. proline-14C in the two proteins of LT was a little less converted to other amino acids than in those of HT. A little higher radioactivity was found in the aspartate and glutamate of the LT protein hydrolysates. The hydroxyproline which is closely related with proline had a little lower level of radioactivity in the LT cell wall. |
| |
| Keywords: | proline-14C metabolism ethanol soluble fractions protein fractions |
|
|
