| Purification of Two Antimicrobial Substances Produced by Bacillus subtilis Strain B11 and Their Properties |
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| 作者姓名： | LI Qi-qin MENG Xian-ying WU Xue LIN Wei DUAN Cheng-jie FENG Jia-xun TANG Ji- liang |
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| 作者单位： | [1]Institute of Plant Pathology, Guangxi University, Nanning 530005, P.R. China [2]Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization/Guangxi University, Nanning 530005, P.R. China [3]College of Life Science and Technology, Guangxi University, Nanning 530005, P.R. China |
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| 摘 要： | Two antimicrobial substances produced by Bacillus subtilis strain B 11 were purified by boiling, DEAE 52 anion exchange chromatography and aluminum oxide adsorption chromatography, These purified substances showed only one spot on silica gel thin layer chromatography （TLC）. Antimicrobial assays showed that antimicrobial substances A and B inhibited the growth of plant pathogens such as Fusarium oxysporum Schl f.sp. niveum, Rhizoctonia solani, Ralstonia solanacearum and Xanthomonas oryzae pv. oryzae. In addition, antimicrobial substance B could also inhibit the growth of Magnaporthe grisea. These two antimicrobial substances were resistant to proteolytic enzymes and temperature as high as 121℃.
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| 关 键 词： | 细菌 土壤 微生物 生态系统 净化 |
| 收稿时间： | 2005-11-05 |
| 修稿时间： | 2006-03-06 |
Purification of Two Antimicrobial Substances Produced by Bacillus subtilis Strain B11 and Their Properties |
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| LI Qi-qin MENG Xian-ying WU Xue LIN Wei DUAN Cheng-jie FENG Jia-xun TANG Ji- liang.Purification of Two Antimicrobial Substances Produced by Bacillus subtilis Strain B11 and Their Properties[J].Agricultural Sciences in China,2006,5(5):363-369. |
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| Authors: | LI Qi-qin MENG Xian-ying WU Xue LIN Wei DUAN Cheng-jie FENG Jia-xun TANG Ji-liang |
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| Institution: | 1. Institute of Plant Pathology, Guangxi University, Nanning 530005, P.R. China
2. Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization/Guangxi University, Nanning 530005, P.R. China;College of Life Science and Technology, Guangxi University, Nanning 530005, P.R. China |
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| Abstract: | Two antimicrobial substances produced by Bacillus subtilis strain B11 were purified by boiling, DEAE 52 anion exchange chromatography and aluminum oxide adsorption chromatography. These purified substances showed only one spot on silica gel thin layer chromatography (TLC). Antimicrobial assays showed that antimicrobial substances A and B inhibited the growth of plant pathogens such as Fusarium oxysporum Schl f.sp. niveum, Rhizoctonia solani, Ralstonia solanacearum and Xanthomonas oryzae pv. oryzae. In addition, antimicrobial substance B could also inhibit the growth of Magnaporthe grisea. These two antimicrobial substances were resistant to proteolytic enzymes and temperature as high as 121°C. |
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| Keywords: | Bacillus subtilis antimicrobial substance purification |
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