| 富集培养促进宏基因组途径获得聚酮酶基因的研究 |
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| 引用本文： | 唐敏,洪葵.富集培养促进宏基因组途径获得聚酮酶基因的研究[J].安徽农业科学,2008,36(17):7159-7161. |
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| 作者姓名： | 唐敏 洪葵 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,海南海口,571101;海南大学农学院,海南儋州,571737
2. 中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 国家自然科学基金
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新世纪优秀人才支持计划 |
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| 摘 要： | 进行了富集培养促进宏基因组途径获得聚酮酶基因的研究。结果表明,通过富集培养土壤样品有效提高了红树林土壤高分子量DNA的提取效率,且经分散差速离心(DDC)法预处理并由RH-土壤浸提液富集培养后,可同时检测到放线菌和聚酮化合物合成酶的特征序列,该富集培养样品有助于构建红树林土壤宏基因组BAC文库和聚酮合酶基因的筛选。
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| 关 键 词： | 聚酮合酶 宏基因组文库 富集培养 红树林土壤 |
| 文章编号： | 0517-6611(2008)17-07159-03 |
| 修稿时间： | 2008年4月14日 |
Acquisition of Polyketide Synthase Genes by Enrichment Culture Through Metagenome Approach |
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| TANG Min.Acquisition of Polyketide Synthase Genes by Enrichment Culture Through Metagenome Approach[J].Journal of Anhui Agricultural Sciences,2008,36(17):7159-7161. |
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| Authors: | TANG Min |
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| Abstract: | Study on the acquisition of polyketide synthase genes by enrichment culture through metagenome approach was carried out.Result] showed that enrichment culture of soil sample effectively improved the extraction efficiency of high molecular weight DNA in mangrove soil.After DDC pretreatment and enrichment culture by RH-soil water extract,feature sequences of polyketide synthase and actinomycete could be detected simultaneously.This enrichment culture sample could help to construct the metagenomic BAC library of mangrove soil and to screening polyketide synthase genes. |
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| Keywords: | Polyketide synthase Metagenomic library Enrichment culture Mangrove soil |
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