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地锦草对水产病原菌体外抗活性的研究
引用本文:田海军.地锦草对水产病原菌体外抗活性的研究[J].水利渔业,2010(1).
作者姓名:田海军
作者单位:信阳农业高等专科学校水产科学系;
摘    要:用95%的乙醇来提取地锦草(Euphorbia humifusa Wild)中的化合物,再分别用石油醚、乙酸乙酯、正丁醇和水对地锦草乙醇提取物进行分极性段萃取,研究地锦草各极性段二甲基亚砜萃取溶液对嗜水气单胞菌(Aeromonas hydrophila)、温和气单胞菌(Aeromonas sobria)、柱状屈挠杆菌(Flexibacter calumnaris)、鳗弧菌(Vibrisis angnillarumz)4种常见水产动物病原菌的抑菌效果。结果表明,地锦草各极性段的抑菌作用强度有明显差别(P<0.05),其中乙酸乙酯、石油醚极性段的抑菌作用最为强烈,对温和气单胞菌的MIC均≥12.5mg/mL;乙酸乙酯极性段对嗜水气单胞菌、鳗弧菌的MIC均≥50mg/mL;石油醚极性段对嗜水气单胞菌、鳗弧菌的MIC≥25mg/mL;正丁醇和水极性段对4种菌的MIC均≥50mg/mL。

关 键 词:地锦草  嗜水气单胞菌  温和气单胞菌  柱状屈挠杆菌  鳗弧菌  

In vitro Activity of Extracts from Euphorbia humifusa Willd on Bacteria in Aquiculture
TIAN Hai-jun.In vitro Activity of Extracts from Euphorbia humifusa Willd on Bacteria in Aquiculture[J].Reservoir Fisheries,2010(1).
Authors:TIAN Hai-jun
Institution:TIAN Hai-jun(Department of Aquatic Science,Xinyang Agricultural College,Xinyang 464000,China)
Abstract:The Euphorbia humifusa Willd was firstly extracted by 95% ethanol,and then extracted by sherwood oil,ethyl acetate,normal butanol and water,respectively. We studied the inhibited activities of different polar extracts from Euphorbia humifusa Willd against the common aquatic pathogenic bacteria such as backwoods coli,Bacillus subtilis,golden yellow staphylococcus and eel vibrio bacteriostasis. The results showed that: the anti-bacteria activity of extracts from Euphorbia humifusa with various extraction exhi...
Keywords:Euphorbia humifusa Willd  Aeromonas hydrophil  Aeromonas sobria  Flexibacter columnaris  Vibrisis angnillarumz  
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