| 樱桃番茄叶体细胞胚发生过程中抗氧化酶活性和生理参数的变化 |
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| 引用本文： | 关正君,郭斌,尉亚辉.樱桃番茄叶体细胞胚发生过程中抗氧化酶活性和生理参数的变化[J].核农学报,2011,25(3):594-601. |
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| 作者姓名： | 关正君 郭斌 尉亚辉 |
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| 作者单位： | 1. 运城学院生命科学系,山西运城044000;西北大学生命科学学院,西部资源生物与现代生物技术省部共建教育部重点实验室,陕西西安710069
2. 西北大学生命科学学院,西部资源生物与现代生物技术省部共建教育部重点实验室,陕西西安710069 |
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| 基金项目： | 陕西省生物技术重点实验室项目，陕西省教育厅专项科研资助项目，国家自然科学基金，陕西省财政专项项目，西北大学自主创新类项目 |
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| 摘 要： | 以樱桃番茄叶片为外植体在附加6-BA 2.0mg·L+IAA 0.1mg·L的MS培养基上诱导体细胞胚发生,研究了樱桃番茄体细胞胚胎发生过程中抗氧化酶活性和某些生理参数的变化.结果表明,在樱桃番茄体细胞胚发生过程中,抗氧化酶活性变化表现为SOD活性在培养第7天和第21天出现2次高峰,分别为73.69U·g-1FW和77...
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| 关 键 词： | 樱桃番茄 体细胞胚发生 可溶性蛋白含量 叶绿素含量 抗氧化酶活性 IAA氧化酶 |
| 收稿时间： | 2010-09-26 |
| 修稿时间： | 2011-01-11 |
CHANGES OF ANTI-OXIDATIVE ENZYMES ACTIVITIES AND PHYSIOLOGICAL PARAMETERS DURING SOMATIC EMBRYOGENESIS OF CHERRY TOMATO |
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| GUAN Zheng-jun,GUO Bin,WEI Ya-hui.CHANGES OF ANTI-OXIDATIVE ENZYMES ACTIVITIES AND PHYSIOLOGICAL PARAMETERS DURING SOMATIC EMBRYOGENESIS OF CHERRY TOMATO[J].Acta Agriculturae Nucleatae Sinica,2011,25(3):594-601. |
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| Authors: | GUAN Zheng-jun GUO Bin WEI Ya-hui |
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| Institution: | 1. Department of Life Sciences, Yuncheng University, Yuncheng, Shanxi 044000;
2. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education,Northwest University, Xi’an, Shaanxi 710069 |
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| Abstract: | The somatic embryogenesis was induced from the leaves of cherry tomato (Lycopersivon esculentum Mill.) on MS medium with 2.0mg ·L-16-BA and 0.1mg·L-1 IAA,and the changes of antioxidant activities and some physiological parameters during somatic embryogenesis were analyzed. Results showed that the activities of antioxidant enzymes were as follows during somatic embryogenesis. Firstly, SOD activity of the callus presented two peaks of 73.69U·g-1FW and 77.76U·g-1FW on the 7th and the 21st cultured days, respectively. And POD activity decreased, then increased to its minimum (0.6317U·g-1min-1FW) on the 21st day. However, CAT activity had no significant change during the whole culture time and the highest value was 23.0798mg·g-1min-1 on the 21st day. At the same time, IAA oxidase activity had the highest value (737.0748μg(IAA)g-1FWh-1) on the 35th day and the lowest value was 122.954 μg(IAA)g-1FWh-1. Furthermore, MDA showed a significant downward trend overall, and H2O2 content increased till the 14th day (the highest value was 11.4522μmol·L-1g-1FW) and then decreased. Changes of the physiological parameters showed that the fresh weight of the callus gradually increased with the extension of culture time, and the changes of soluble protein content had a peak value 0.7299mg·g-1FW on the 35th day. The highest value of chlorophyll content was 1.5891mg·g-1FW on the 28th day. Besides, the change of electrical conductivity had a maximum (25.8%) on the 14th day. It indicated that POD played an important role in somatic embryogenesis of cherry tomato leaf explants. At the same time, soluble protein content and cell membrane permeability were correlated with process of somatic embryogenesis; the activities of SOD and IAA oxidase as well as H2O2 accumulation had regular changes, which closely related to the development of embryonic cells. |
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| Keywords: | cherry tomato somatic embryogenesis soluble protein content chlorophyll content antioxidant activities IAA oxidase activity |
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