| 鹿流行性出血病病毒高通量液相芯片检测方法的建立 |
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| 引用本文： | 詹爱军,王新卫,陈书琨,孙洁,陈兵,阮周曦,花群义.鹿流行性出血病病毒高通量液相芯片检测方法的建立[J].动物医学进展,2010,31(2):36-40. |
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| 作者姓名： | 詹爱军 王新卫 陈书琨 孙洁 陈兵 阮周曦 花群义 |
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| 作者单位： | 1. 深圳出入境检验检疫局动植物检验检疫技术中心,广东深圳,518010
2. 河南农业大学牧医工程学院,河南郑州,450002 |
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| 基金项目： | 科技部“863”计划项目(2006AA10Z445);;国家质检总局资助项目(2008IK011) |
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| 摘 要： | 为建立可检测鹿流行性出血病病毒(EHDV)的液相芯片快速检测技术,用DNAStar软件对GenBank中EHDV的VP7基因进行序列分析,设计EHDV特异性探针并用生物素标记,与荧光编码微球偶联后与病毒VP7基因的PCR产物杂交反应,用液相芯片检测仪(Liquichip200)检测荧光信号建立了EHDV快速高通量液相芯片检测方法。检测结果显示,该法具有较好的特异性,不与其他虫媒病病毒反应;检测灵敏度为100个TCID50。建立了快速检测EHDV的液相芯片技术,为进一步搭建EHDV快速高通量检测平台奠定了基础。
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| 关 键 词： | 流行性出血病痛毒 液相芯片 检测 |
Development of Liquichip Detection Technique for Detecting Epizootic Hemorrhagic Disease Virus of Deer |
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| ZHAN Ai-jun,WANG Xin-wei,CHEN Shu-kun,SUN Jie,CHEN Bing,RUAN Zhou-xi,HUA Qun-yi.Development of Liquichip Detection Technique for Detecting Epizootic Hemorrhagic Disease Virus of Deer[J].Progress In Veterinary Medicine,2010,31(2):36-40. |
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| Authors: | ZHAN Ai-jun WANG Xin-wei CHEN Shu-kun SUN Jie CHEN Bing RUAN Zhou-xi HUA Qun-yi |
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| Institution: | 1.Technical Center of Animal and Plant Quarantine Inspection;Shenzhen CIQ;Shenzhen;Guangdong;518010;China;2. College of Animal Husbandry and Veterinary Medicine;Henan Agricultural University;Zhengzhou;Henan;450002;China |
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| Abstract: | To develop liquichip method for detecting epizootic haemorrhagic disease virus of deer,the VP7 gene of EHDV published in the GenBank was analyzed by using the software DNAStar 5.0,and specific EHDV probe labeled with biotin was prepared and coupled with fluorescence-coded microspheres,which was used for hybridization reaction to RT-PCR products of EHDV. Liquichip detection method for detecting EHDV was established by using Liquichip 200 to detect fluorescence signal in the reaction system. The results demon... |
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| Keywords: | Epizootic haemorrhagic disease virus liquichip detection |
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