Eradication of PVS from potato clones through excision of meristems from in vitro,heat-treated shoot tips |
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| Authors: | C R Brown S Kwiatkowski M W Martin P E Thomas |
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| Institution: | 1. Irrigated Agriculture Research and Extension Center, Agricultural Research Service, U.S. Department of Agriculture, 99350, Prosser, WA
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| Abstract: | In vitro shoot tips from PVS-infected potato breeding clones and cultivars were exposed to a constant temperature of 34 C and 8,100 lux light. Meristems were excised from heat-treated shoot tips after 3 to 7 weeks of heat therapy. Serological tests were performed on shoot tips derived from meristems excised from heat-treated shoot tips. Additional serological tests, combined with bioassays, applied to six-week-old pot-grown cuttings derived from these shoot tips, did not detect PVS in 19 out of 21 clones given heat therapy. A 90% rate of eradication was achieved. |
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