| 菊芋基因组DNA提取方法的比较 |
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| 引用本文： | 赵孟良,韩睿,马胜超,李莉.菊芋基因组DNA提取方法的比较[J].中国蔬菜,2012,1(14):41-44. |
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| 作者姓名： | 赵孟良 韩睿 马胜超 李莉 |
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| 作者单位： | 青海大学农林科学院，青海省蔬菜遗传与生理重点实验室，青海西宁 810016 |
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| 基金项目： | 青海省蔬菜遗传与生理重点实验室资助项目(Sczdsys-2011-02) |
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| 摘 要： | 采用改良CTAB法、改进的高盐SDS法及两种植物基因组DNA提取试剂盒法等４种方法提取菊芋基因组DNA，并进行电泳分析、质量检测及ISSR引物扩增检测。结果表明，改良CTAB法优于其他3种方法，提取的DNA质量较好、纯度较高，能够满足ISSR-PCR扩增要求。
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| 关 键 词： | 菊芋 基因组DNA 提取方法 |
Comparison of Genomic DNA Extraction from Helianthus tuberosus L |
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| ZHAO Meng-liang
,
HAN Rui
,
MA Sheng-chao
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LI Li.Comparison of Genomic DNA Extraction from Helianthus tuberosus L[J].China Vegetables,2012,1(14):41-44. |
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| Authors: | ZHAO Meng-liang HAN Rui MA Sheng-chao LI Li |
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| Institution: | Qinghai University of Agriculture and Forestry Research，Qinghai Province Key Laboratory of Vegetable Genetics and Physiology，Xining 810016，Qinghai，China |
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| Abstract: | The genomic DNA was isolated from the leaf of Helianthus tuberosus L.by the improved CTAB method,improved high salt SDS method and 2 genomic DNA kits.The DNA quality was examined by agarose gel electrophoresis and PCR amplification of ISSR.The result showed that the improved CTAB method was obviously better than the others.The DNA sample obtained by the improved CTAB method was pure and had good quality,which could satisfy the need for further ISSR analysis. |
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| Keywords: | Helianthus tuberosus L Genomic DNA Extraction method |
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