| 转rolA、B、C基因枳橙快繁技术 |
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| 引用本文： | 胡春华,谢玉明,黄训才,郭琛,焦徕,吴芯茹,邓子牛.转rolA、B、C基因枳橙快繁技术[J].果树学报,2006,23(1):142-144,F0004. |
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| 作者姓名： | 胡春华 谢玉明 黄训才 郭琛 焦徕 吴芯茹 邓子牛 |
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| 作者单位： | 湖南农业大学园艺园林学院,长沙,410128 |
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| 基金项目： | 国家转基因植物专项基金
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湖南农业大学校科研和教改项目 |
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| 摘 要： | 通过对转rol基因枳橙B、D、E系及对照的高接植株春梢茎段进行萌芽诱导、增殖及试管苗生根成苗的试验,摸索出适宜枳橙快繁各种培养基配方及培养条件。结果表明,以MS+6-BA1mg/L的培养基适合于芽萌发,以MS+6-BA0.5mg/L+NAA0.05mg/L的培养基适合于芽增殖,生根则以1/2MS+NAA0.5mg/L+0.2%活性炭的组合最佳,转基因各系生根率均在93.3%以上,显著高于对照生根率(66.7%)。快繁苗移栽成活率可达90%,目前生长良好。
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| 关 键 词： | rolABC基因 转基因枳橙 组织培养 快速繁殖 |
| 文章编号： | 1009-9980（2006）01-142-03 |
| 收稿时间： | 2005-04-11 |
| 修稿时间： | 2005-04-112005-11-18 |
In vitro propagation of rol A, B, C transgenic citrange |
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| HU Chun-hua,XIE Yu-ming,HUANG Xun-cai,GUO Chen,JIAO Lai,WU Xin-ru,DENG Zi-niu.In vitro propagation of rol A, B, C transgenic citrange[J].Journal of Fruit Science,2006,23(1):142-144,F0004. |
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| Authors: | HU Chun-hua XIE Yu-ming HUANG Xun-cai GUO Chen JIAO Lai WU Xin-ru DENG Zi-niu |
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| Institution: | Horticulture and Landscape College, Hunan Agricultural University, Changsha, Hunan 410128 China |
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| Abstract: | In vitro propagation makes it possible to speed the multiplication of the rol A,B,C transgenic citrange plants, so it is possible to quickly evaluate this new rootstock. Using bud sticks of the transgenic clones (B, D, E) and the control citrange as explants, culture media containing various plant growth regulators were tested for bud burst, shoot multiplication and rooting. The results indicated that MS medium containing 1 mg/L 6-BA resulted in fastest bud bursting, MS added with 6-BA 0.5 mg/L NAA 0.05 mg/L was the best choice for shoot multiplication, and 1/2MS supplied with NAA 0.5 mg/L and 0.2% activated charcoal showed quite suitable for rooting. The transgenic clones had significantly higher rooting percentage (93.3%) than that of the control (66.7%). |
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| Keywords: | rol A B C genes Transgenic citrange Tissue culture Micropropagation |
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