| 表皮生长因子对水牛胚胎体外发育及凋亡的影响 |
| |
| 引用本文： | 郑海英,杨炳壮,杨春艳,庞春英,黄芬香,尚江华,李日聪,张秀芳,覃广胜,梁贤威.表皮生长因子对水牛胚胎体外发育及凋亡的影响[J].畜牧与兽医,2011,43(6). |
| |
| 作者姓名： | 郑海英 杨炳壮 杨春艳 庞春英 黄芬香 尚江华 李日聪 张秀芳 覃广胜 梁贤威 |
| |
| 作者单位： | 中国农业科学院广西水牛研究所,广西南宁530001;广西水牛遗传繁育重点实验室,广西南宁530001 |
| |
| 基金项目： | 农业部948项目，科技部国际合作项目，广西科技攻关项目，基本科研业务费项目 |
| |
| 摘 要： | 为了探讨表皮生长因子(EGF)对水牛早期胚胎体外发育及凋亡的影响,通过收集屠宰场卵巢卵母细胞进行体外成熟和体外受精,将假定的受精卵置于含不同浓度EGF(0,25,50和100 ng/mL)的培养液中培养,检查分裂率和囊胚发育率,用细胞凋亡试剂盒(Annexin-V-FluosStaining kit)试剂染色,统计囊胚细胞凋亡率和坏死率。结果表明:50 ng/mL EGF组的孵化囊胚率显著高于对照组(P<0.05),该组细胞凋亡率和坏死率显著低于对照组(P<0.05)。100 ng/mL EGF的卵裂率、囊胚率、D7囊胚率和孵化囊胚率显著低于对照组和其他试验组(P<0.05)。细胞凋亡率和坏死率显著高于其他各组(P<0.05)。提示:一定浓度的EGF可提高囊胚孵化率,并可抑制胚胎细胞的凋亡。
|
| 关 键 词： | 水牛 胚胎 表皮生长因子 发育 凋亡 |
Effect of epidermal growth factor on development and apoptosis of buffalo embryo in vitro |
| |
| ZHENG Hai-ying,YANG Bing-zhuang,YANG Chun-yan,PANG Chun-ying,HUANG Fen-xiang,SHANG Jiang-hua,LI Ri-cong,ZHANG Xiu-fang,QIN Guang-sheng,LIANG Xian-wei.Effect of epidermal growth factor on development and apoptosis of buffalo embryo in vitro[J].Animal Husbandry & Veterinary Medicine,2011,43(6). |
| |
| Authors: | ZHENG Hai-ying YANG Bing-zhuang YANG Chun-yan PANG Chun-ying HUANG Fen-xiang SHANG Jiang-hua LI Ri-cong ZHANG Xiu-fang QIN Guang-sheng LIANG Xian-wei |
| |
| Institution: | ZHENG Hai-ying1,2,YANG Bing-zhuang1,YANG Chun-yan1,PANG Chun-ying1,HUANG Fen-xiang1,SHANG Jiang-hua1,LI Ri-cong1,ZHANG Xiu-fang1,QIN Guang-sheng1,LIANG Xian-wei1,2(1.Guangxi Buffalo Research Institute,Chinese Academy of Agricultural Science,Nanning 530001,China,2.Guangxi Key Laboratory of Buffalo Genetics,Breeding and Reproduction,China) |
| |
| Abstract: | To evaluate the influence of epidermal growth factor(EGF) on buffalo early embryo development and apoptosis in vitro,oocytes from buffalo ovaries at a slaughterhouse were matured and fertilized,and presumptive zygotes were cultured in the medium supplemented with EGF(0,25,50 or 100 ng/mL),then examined cleavage and blastocyst rate,respectively.After staining with Annexin V-Fluos Apoptosis Derection Kit(Annexin-V-Fluos Staining kit),apoptosis and necrosis of blastocyst under fluorescent microscope was examin... |
| |
| Keywords: | buffalo embryo epidermal growth factor development apoptosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
