| “桐优1”等泡桐优良无性系组培高效繁育体系建立 |
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| 引用本文： | 江香梅,戴小英,温强,杨书斌,李秋英.“桐优1”等泡桐优良无性系组培高效繁育体系建立[J].江西林业科技,2009(2). |
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| 作者姓名： | 江香梅 戴小英 温强 杨书斌 李秋英 |
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| 作者单位： | 1. 江西省林业科学院省植物生物技术技术重点实验室,江西,南昌,330032
2. 抚州市林业科学研究所,江西,临川,344000 |
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| 基金项目： | 江西省科技厅农业重点攻关项目 |
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| 摘 要： | 采用抚州市林业科学研究所和江西省林业科学院选育、江西省林木良种审定委员会认定的"桐优1"等3个泡桐优良无性系为繁殖材料,对无菌系建立外植体筛选、外植体诱导、分化、增殖、组培苗生根等条件进行了较系统的优化试验,结果表明:以根萌条为外植体是建立无菌系的理想材料;外植体诱导培养基以MS+BA2.0mg/L(单位下同)+NAA0.2+2,4D0.1为好;分化和增殖培养基以MS+BA3.0+IBA0.3;生根培养基以MS+IBA0.1为好。为降低育苗成本,培养基中的碳源用食用白糖(30g/L),固化物用卡拉胶(7g/L)。本试验通过优化筛选,建立了一套经济、高效的组培快速繁殖体系,为泡桐组培规模化生产奠定了良好的物质和技术基础。
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| 关 键 词： | 泡桐 “桐优1” 优良无性系 组培繁殖 |
Establishment of High Effect Regeneration System of Tissue Culture for Three Improved Clones in Paulownia fortunei |
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| JIANG Xiangmei,YANG Shubin,DAI Xiaoying,WEN Qiang,LI Qiuying.Establishment of High Effect Regeneration System of Tissue Culture for Three Improved Clones in Paulownia fortunei[J].Jiangxi Forestry Science and Technology,2009(2). |
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| Authors: | JIANG Xiangmei YANG Shubin DAI Xiaoying WEN Qiang LI Qiuying |
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| Institution: | 1.Jiangxi Provincial Bio-tech Key Lab for Plant;Jiangxi Academy of Forestry;Nanchang Jiangxi 330032;China;2.Fuzhou City Forestry Research Institute;Linchuan Jiangxi 344000;China |
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| Abstract: | With three improved clones just as "Tongyou1"et al.select-cultivated by Fuzhou City Forestry Research Institute and Jiangxi Academy of Forestry,convinced by Jiangxi Forest Tree Breeding Examining and Approving Committee for propagation materials,the optimization conditions for explants selection,adventitious bud induction,differentiation,propagation and rooting were tested systematically in this paper.The results demonstrated that the ideal explants materials for sterilization clones construction were shoot... |
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| Keywords: | Paulownia fortunei "Tongyou1" Improved clones Tissue culture |
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