| 野生细叶结缕草再生体系的建立 |
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| 引用本文： | 张瑜,杨知建,张志扬,张志飞.野生细叶结缕草再生体系的建立[J].湖南农业大学学报(自然科学版),2007,33(1):105-108. |
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| 作者姓名： | 张瑜 杨知建 张志扬 张志飞 |
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| 作者单位： | 1. 湖南农业大学,农学院,湖南,长沙,410128
2. 湖南农业大学,作物基因工程湖南省重点实验室,湖南,长沙,410128 |
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| 摘 要： | 以适合南方地区夏季种植的采自泰国北部的一种野生的细叶结缕草为供试材料，以匍匐茎节为外植体建立其再生体系．结果表明：在MS基本培养基上添加5．0mg／L2，4-D和NB培养基上添加0．2mg／LIAA所产生的愈伤组织最好，而添加2．0raM-2，4-D＋1．0mg／LBA时分化效果最好，1／2MS＋0．5mg／LNAA培养基最适于生根．
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| 关 键 词： | 野生细叶结缕草 组织培养 诱导 分化 |
| 文章编号： | 1007-1032(2007)01-0105-04 |
| 修稿时间： | 2006年12月27 |
Foundation of regenerative system of wild Zoysia tenuifolia |
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| ZHANG Yu,YANG Zhi-jian,ZHANG Zhi-yang,ZHANG Zhi-fei.Foundation of regenerative system of wild Zoysia tenuifolia[J].Journal of Hunan Agricultural University,2007,33(1):105-108. |
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| Authors: | ZHANG Yu YANG Zhi-jian ZHANG Zhi-yang ZHANG Zhi-fei |
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| Abstract: | Zoysia tenuifolia was a warm-season grass character of which was fine for building lawn.Using a kind of wild thin Z.tenuifolia growing in the northern area of Thailand and the crawal stem as experiment material,the regenerative system was founded.The result showed:adding 5.0 mg/L concentration of 2,4-D to the MS basic medium and adding 0.2 mg/L concentration of IAA to the NB basic medium by to the best callus tissue could be induced,while,2.0 mg/L 2,4-D 1.0 mg/L BA was optimal for the differentiation of callus tissue,1/2MS 0.5 mg/L NAA was the best radication medium. |
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| Keywords: | wild Zoysia tenuifolia tissue culture inducement differentiation |
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