| SLE患者CD4+CD25+调节性T细胞及Foxp3基因表达的研究 |
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| 引用本文： | 唐蓉,唐德燊,伍昌林.SLE患者CD4+CD25+调节性T细胞及Foxp3基因表达的研究[J].湛江医学院学报,2006,24(4):346-348. |
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| 作者姓名： | 唐蓉 唐德燊 伍昌林 |
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| 作者单位： | 广东医学院附属医院肾内科 广东湛江524001 |
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| 摘 要： | 目的:研究系统性红斑狼疮(SLE)患者外周血CD 4 CD 25 调节性T细胞及Foxp3基因的表达水平,了解它们在SLE发病机制中的作用。方法:分别收集25例SLE患者(SLE组)及健康人(对照组)外周抗凝静脉血,分离纯化T淋巴细胞。PE标记抗CD 4单抗,F ITC标记的抗CD 25单抗,作双色流式细胞术,分析SLE患者外周血CD 4 CD 25 调节性T细胞百分率,RT-PCR检测T细胞Foxp3 mRNA表达。结果:SLE组外周血CD 4 T、CD 4 CD 25 T细胞百分率及T细胞Foxp3 mRNA水平均低于对照组(P<0.01),并且CD 4 CD 25 T细胞百分率与Foxp3mRNA水平呈依赖关系(P<0.01)。结论:SLE患者外周血存在细胞免疫功能失调,CD 4 CD 25 调节性T细胞数量减少和Foxp3mRNA表达下调可能与SLE的免疫学发病机制有关。
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| 关 键 词： | 系统性红斑狼疮 调节性T细胞 Foxp3基因 |
| 文章编号： | 1005-4057（2006）04-0346-03 |
| 收稿时间： | 2006-04-06 |
| 修稿时间： | 2006-05-08 |
Expression of CD4+CD25+ regulatory T cell and Foxp3 gene in systemic lupus erythematosus |
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| TANG Rong,TANG De-shen,WU Chang-lin.Expression of CD4+CD25+ regulatory T cell and Foxp3 gene in systemic lupus erythematosus[J].Journal of Guangdong Medical College,2006,24(4):346-348. |
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| Authors: | TANG Rong TANG De-shen WU Chang-lin |
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| Abstract: | Objective: To observe the expression of CD4~+CD25~+ regulatory T cell and Foxp3 gene from peripheral blood in patients with systemic lupus erythematosus(SLE),and to investigate their roles in the pathogenesis of SLE.Methods: The percentage of CD4~+CD25~+ regulatory T cells was detected by bicolor flow cytometry,and the expression of Foxp3 mRNA on T lymphocytes was determined by RT-PCR in 25 SLE patients and 25 healthy controls.Results: The percentage of CD4~+ cells and CD4~+CD25~+ T cells,and level of Foxp3 mRNA on T lymphocytes were significantly lower in SLE group than those in control group(P<0.01).There was a positive correlation between the percentage of CD4~+CD25~+ T cell expression and expression of Foxp3 mRNA(r=0.786,P<0.01) in SLE group.Conclusion:There is a disturbance of cellular immune function in SLE.The downexpression of CD4~+CD25~+ regulatory T cell and Foxp3 mRNA on T lymphocytes may be involved in the immunopathogenesis of SLE. |
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| Keywords: | systemic lupus erythematosus regulatory T cell t Foxp3 gene |
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