| 灰枣红点软腐病病原菌的鉴定 |
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| 引用本文： | 牛晓科,崔建州,侯晓杰,陈谟林,冉隆贤.灰枣红点软腐病病原菌的鉴定[J].植物病理学报,2010,40(2):217-221. |
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| 作者姓名： | 牛晓科 崔建州 侯晓杰 陈谟林 冉隆贤 |
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| 作者单位： | 1 河北农业大学林学院, 保定 071001;2 新郑市枣树科学研究所, 新郑 451150 |
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| 基金项目： | 教育部科学技术研究重点项目，河北农业大学科研基金 |
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| 摘 要： | The damage and symptoms of jujube fruit soft rot in Xinzheng, Henan Province, were investigated from 2006 to 2008, and the pathogen was identified based on the morphological characteristics and the ITS se-quence of ribosome DNA. The results showed that the aerial mycelium of colony was white in color in the first four days, then turned gray after incubation on PDA for 5-6 d at 25℃ and became black two weeks later. The mycelia grew luxuriantly with velvet character. The pycnidium was flask-shaped with a height of 196.9μm and a width of 213.3μm on the avereage. The conidium was colorless with single cell and had the shape of spindle, its size was (15.0-20.0)μm× (4.5-6.5)μm. The conidiophore was fastigiate. The homology of ITS sequence of ribosome DNA between the tested strain NXK and Botryosphaeria dothidea (GenBank ac-cession number:AJ938005) reached 99.87%, with a difference of only two base pairs. Based on the results of both morphological characters and molecular identification, the pathogen of jujube fruit soft rot in Xinzheng was identified as B. dothidea (Moug. ex Fr.) Ces. et de Not..
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| 关 键 词： | 软腐病菌 灰枣 培养基 核糖体 形态特征 分生孢子器 葡萄座腔菌属 致病性测定 分离菌株 病原菌鉴定 |
Identification of the pathogen causing fruit soft rot in Ziziphus jujuba Mill. |
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| NIU Xiao-ke,CUI Jian-zhou,HOU Xiao-jie,CHEN Mo-lin,RAN Long-xian.Identification of the pathogen causing fruit soft rot in Ziziphus jujuba Mill.[J].Acta Phytopathologica Sinica,2010,40(2):217-221. |
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| Authors: | NIU Xiao-ke CUI Jian-zhou HOU Xiao-jie CHEN Mo-lin RAN Long-xian |
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| Institution: | 1 Forestry College, Agricultural University ofHebei, Baoding 071001, China;2 Jujuba Institute of Xinzheng, Xinzheng 451150, China |
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| Abstract: | The damage and symptoms of jujube fruit soft rot in Xinzheng, Henan Province, were investigated from 2006 to 2008, and the pathogen was identified based on the morphological characteristics and the ITS se-quence of ribosome DNA. The results showed that the aerial mycelium of colony was white in color in the first four days, then turned gray after incubation on PDA for 5-6 d at 25℃ and became black two weeks later. The mycelia grew luxuriantly with velvet character. The pycnidium was flask-shaped with a height of 196.9μm and a width of 213.3μm on the avereage. The conidium was colorless with single cell and had the shape of spindle, its size was (15.0-20.0)μm× (4.5-6.5)μm. The conidiophore was fastigiate. The homology of ITS sequence of ribosome DNA between the tested strain NXK and Botryosphaeria dothidea (GenBank ac-cession number:AJ938005) reached 99.87%, with a difference of only two base pairs. Based on the results of both morphological characters and molecular identification, the pathogen of jujube fruit soft rot in Xinzheng was identified as B. dothidea (Moug. ex Fr.) Ces. et de Not.. |
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| Keywords: | Ziziphus jujuba morphological characteristics ITS sequence ribosome DNA Botryosphaeria dothidea(Moug ex Fr ) Ces et de Not |
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