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| “胰岛素-转铁蛋白-硒钠”对山羊卵母细胞体外成熟及胚胎发育的影响 | |
| 引用本文： | 刘凤军,张玉玲,杨自军,王国华,张涌.“胰岛素-转铁蛋白-硒钠”对山羊卵母细胞体外成熟及胚胎发育的影响[J].安徽农业科学,2008,36(28). |
| 作者姓名： | 刘凤军 张玉玲 杨自军 王国华 张涌 |
| 作者单位： | 1. 河南科技大学动物科技学院,河南洛阳,471003;西北农林科技大学生物工程研究所,陕西杨凌,712100 2. 河南科技大学动物科技学院,河南洛阳,471003 3. 西北农林科技大学生物工程研究所,陕西杨凌,712100 |
| 基金项目： | 国家高技术研究发展计划(863计划)，河南科技大学博士基金 |
| 摘 要： | 目的]为提高山羊卵母细胞及胚胎体外培养的效率,探索无血清体外培养体系。方法]在目前卵母细胞体外成熟及胚胎培养所用的常规培养液中添加1%ITS(胰岛素-转铁蛋白-硒钠)或以1%ITS分别替代2种培养液中FBS,对山羊卵母细胞及孤雌激活胚胎进行体外培养,检测ITS对其发育率的影响。结果]添加ITS到卵母细胞成熟液中,对卵母细胞成熟率没有明显提高,但显著提高了其激活后孤雌胚胎的囊胚率(58.06%vs.48.19%);以ITS替代成熟液中FBS,取得了与FBS组相似的成熟率、卵裂率和囊胚率。添加ITS到胚胎培养液中,显著提高了山羊孤雌胚胎的囊胚率(68.30%vs.56.82%);以ITS替代胚胎培养液中FBS,卵裂率与FBS组无显著差异,囊胚率显著低于FBS组(42.33%vs.56.82%)。结论]ITS对山羊卵母细胞体外成熟及胚胎早期发育均有促进作用;另外,ITS可以替代卵母细胞成熟培养液中的血清,作为无血清培养体系用于相关研究。 |
| 关 键 词： | 胰岛素-转铁蛋白-硒钠 无血清培养 卵母细胞 胚胎 孤雌激活 山羊 |
Effect of Insulin-Transferrin-Selenium on Goat Oocytes Maturation and Embryo Development |
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| Abstract: | Objective] The research aimed to enhance culture efficiencies of oocyte and embryo of goat in vitro and to explore serum-free culture system in vitro.Method] At present,the conventional solutions of oocyte maturation and embryo development in vitro were always added into 1% ITS(Insulin-transferrin-selenium) or using 1% ITS to replace FBS in 2 kinds culture solutions for conducting in vitro cultures of goat oocyte and parthenogenetic embryo.The influences of ITS on their developments were detected.Result] ITS in maturation liquid of oocytes could not increase oocytes maturation rate but significantly increased blastocyst rate(58.06% vs.48.19%)of parthenogenetic embryo.If FBS in maturation liquid of oocytes was replaced by ITS,the maturation rate,cleavage rate and blastocyst rate were basically unchanged.Adding ITS into embryo medium could increase blastocyst rate(68.30% vs.56.82%)of parthenogenetic embryo of goat.If FBS in embryo medium was replaced by ITS,the cleavage rate didn't change basically,while the blastocyst rate in ITS was obviously lower than that in FBS group(42.33% vs.56.82%).Conclusion] ITS could promote maturation of oocyte in vitro and early embryonic development,in addition,ITS could replace serum in maturation medium of oocyte as serum-free culture system for conducting relevant researches. |
| Keywords: | Insulin-transferrin-selenium Free-serum culture Oocyte Embryo Parthenogenesis Goat |
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