Emasculation of Tartary buckwheat (<Emphasis Type="Italic">Fagopyrum tataricum</Emphasis> Gaertn.) using hot water |
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| Authors: | Yuji Mukasa Tatsuro Suzuki Yutaka Honda |
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| Institution: | (1) National Agricultural Research Center for Hokkaido Region, NARO, Shinsei, Memuro, Kasai Hokkaido, 082-0071, Japan |
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| Abstract: | Hot water treatment was applied to Tartary buckwheat (var. ‘Hokkai T8’) for emasculation. Apical clusters of flower buds were
soaked in a constant temperature water bath (42–44°C) in the afternoon and then the flowers which opened in the next morning
were used for the investigation of self-fertilization and the artificial pollination. Hand pollination was carried out by
rubbing the anthers taken from the male parent (var. ‘Hokkai T10’) to the stigma of the flowers treated by hot water. The
seed set by self-fertilization was almost 0% when treated at 42°C for 8 min or more, at 43°C for 5 min or more and at 44°C
for 3 min or more. With artificial pollination, the best performance was obtained when treated at 44°C for 3 min, in which
the seed set was 55.1% and the matured seed was obtained 37.7% of the hand-pollinated flowers. Nine out of 26 progeny seedlings
emerged and the cotyledonal color of them were all reddish green, probably indicating the hybrid of ‘Hokkai T8’ and ‘Hokkai
T10’. The hybridization was reconfirmed by the segregation in F2. The hot water treatment at 44°C for 3 min was available to other seven varieties. From 24.5% to 100% of the hand-pollinated
flowers set mature seeds though only one self-fertilized seed remained without artificial pollinations. This hot water treatment
must be practically useful in hybridization breeding of Tartary buckwheat. |
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| Keywords: | Emasculation Hot water Tartary buckwheat |
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