| 野生黄花苜蓿组织培养及再生体系研究 |
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| 引用本文： | 侯永霞,王俊杰,赵彦,云锦凤,陈雪英.野生黄花苜蓿组织培养及再生体系研究[J].草地学报,2012,20(3):524-529. |
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| 作者姓名： | 侯永霞 王俊杰 赵彦 云锦凤 陈雪英 |
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| 作者单位： | 内蒙古农业大学生态环境学院 内蒙古自治区草品种育繁工程技术研究中心 草地资源教育部重点实验室, 内蒙古 呼和浩特 010019 |
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| 基金项目： | 国家"863"课题，国家自然科学基金，内蒙古自然科学基金 |
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| 摘 要： | 以呼伦贝尔草原野生黄花苜蓿(Medicago falcata L.)无菌苗的子叶和下胚轴为外植体,对其组织培养及再生体系进行系统的研究,以期为其下一步转基因试验提供良好的受体。结果表明:最佳愈伤诱导培养基为MS+1.5 mg·L-1 2,4-D+0.4 mg·L-1 6-BA+3%蔗糖+0.7%琼脂,下胚轴和子叶的最佳愈伤诱导率均可达到100%。最佳胚性愈伤形成培养基为MS+0.5 mg·L-1 KT+0.1 mg·L-1 NAA+2%蔗糖+0.7%琼脂,胚性愈伤形成率为81.5%;最佳分化芽形成培养基为MS+0.25 mg·L-1 KT+0.05 mg·L-1 NAA+2%蔗糖+0.7%琼脂,分化芽形成率为36.5%。最佳生根培养基为1/2MS+0.5 mg·L-1 NAA+1.5%蔗糖+0.7%琼脂,生根率为93.3%。愈伤诱导结果显示,供试黄花苜蓿材料个体间组织培养再生性存在较大差异,在同一激素水平上有大约1/3植株的愈伤状态优于其他植株。
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| 关 键 词： | 黄花苜蓿 组织培养 再生体系 愈伤 |
| 收稿时间： | 2011-11-11 |
Tissue Culture and Regeneration System of Native Medicago falcata L. |
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| HOU Yong-xia
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WANG Jun-jie
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ZHAO Yan
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YUN Jin-feng
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CHEN Xue-ying.Tissue Culture and Regeneration System of Native Medicago falcata L.[J].Acta Agrestia Sinica,2012,20(3):524-529. |
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| Authors: | HOU Yong-xia WANG Jun-jie ZHAO Yan YUN Jin-feng CHEN Xue-ying |
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| Institution: | College of Ecology and Environmental Science, Inner Mongolia Agricultural University; Inner Mongolia Engineering Technology Research Center for Forage Breeding and Reproduction;The Ministry of Education Key Laboratory of Grassland Resources, Huhhot, Inner Mongolia 010019, China |
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| Abstract: | Using cotyledons and hypocotyls of wild Medicago falcata L.from Hulunbeier grassland as explants,tissue culture and regeneration system of Medicago falcata L.were studied for further transgenic experiments providing a good receptor.Results showed that the optimal medium for callus induction was MS+1.5 mg·L-1 2,4-D+0.4 mg·L-1 6-BA+3% sucrose+0.7% agar,the highest callus induction ratio was 100%.The optimal medium for embryogenic callus was MS+0.5 mg·L-1 KT+0.1 mg·L-1 NAA+2% sucrose+0.7% agar,inducted ratio of embryogenic callus was 81.5%.The optimal medium for differentiated buds was MS+0.25 mg·L-1 KT+0.05 mg·L-1 NAA+2% sucrose+0.7% agar,differentiated ratio of buds was 36.5%.The optimal rooting medium was 1/2MS+0.5 mg·L-1 NAA+1.5% sucrose+0.7% agar,rooting ratio was 93.3%.Callus induction results showed that the tissue culture of Medicago falcata L.had different induction ratio between individuals with the same hormone. |
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| Keywords: | Medicago falcata L Tissue culture Regeneration system Callus |
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