| 不同消毒方式对假俭草种子愈伤诱导的影响 |
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| 引用本文： | 刘明稀,郭振飞.不同消毒方式对假俭草种子愈伤诱导的影响[J].草地学报,2012,20(2):383-388. |
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| 作者姓名： | 刘明稀 郭振飞 |
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| 作者单位： | 1. 湖南农业大学, 湖南 长沙 410128;2. 华南农业大学生命科学学院, 广东 广州 510642;3. 华南农业大学广东省森林植物种质创新与利用重点实验室, 广东 广州 510642 |
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| 基金项目： | 广东省科技计划项目(2006B20201035);湖南农业大学校青年科学基金(2010QN30)资助 |
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| 摘 要： | 为确定假俭草(Eremochloa ophiuroides)再生体系中种子外植体的消毒方式,经过预备试验后共设计A,B,C,D 共4组2系列8种消毒方式。A1:3.5%次氯酸钠消毒25 min,A2:浓硫酸灼烧30 s+3.5%次氯酸钠消毒20 min,B1:7%次氯酸钠消毒20 min,B2:浓硫酸灼烧30 s+7%次氯酸钠消毒20 min,C1:0.05%升汞消毒20 min,C2:浓硫酸灼烧30 s+ 0.05%升汞消毒15 min,D1:0.1%升汞消毒15 min,D2:浓硫酸灼烧30 s+ 0.1%升汞消毒10 min。结果表明:A2的愈伤诱导效果最好,达到52%;D1的愈伤诱导率最低,仅17.33%,其他处理的愈伤诱导率介于二者之间。发现浓硫酸灼烧假俭草种子30 s,可显著提高出愈率;试验证实消毒剂次氯酸钠比升汞有利于假俭草种子的愈伤诱导。
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| 关 键 词： | 假俭草 消毒方式 愈伤诱导 |
| 收稿时间： | 2011-11-21 |
Effects of Different Sterilization Methods on Callus Induction of Centipedegrass (Eremochloa ophiuroides ) |
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| LIU Ming-xi
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GUO Zhen-fei.Effects of Different Sterilization Methods on Callus Induction of Centipedegrass (Eremochloa ophiuroides )[J].Acta Agrestia Sinica,2012,20(2):383-388. |
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| Authors: | LIU Ming-xi GUO Zhen-fei |
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| Institution: | 1. Hunan Agricultural University, Changsha, Hunan Province 410128, China;2. College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong Province 518020, China;3. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, Guangdong Province 510642, China |
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| Abstract: | An efficient regeneration system is crucial to crop breeding by biotechnology.Explant sterilization methods are key factors for an efficient regeneration system.Eight sterilization methods(A1:3.5% sodium hypochlorite 25 min,A2:concentrated sulphuric acid 30 s+3.5% sodium hypochlorite 20 min,B1:7% sodium hypochlorite 20 min,B2:concentrated sulphuric acid 30 s+7% sodium hypochlorite 20 min,C1:0.05% mercury bichloride 20 min,C2:mercury bichloride 30 s+0.05% mercury bichloride 15 min,D1:0.1% mercury bichloride 15 min,D2:mercury bichloride 30 s+0.1% mercury bichloride 10 min) were tested to determine an efficient regeneration system for Centipede grass.Results showed the A2 treatment with 52% callus induction ratio as the best for Centipede grass,whereas the D1 treatment resulted in the lowest callus induction ratio(17.33%).Seeds treated by concentrated sulfuric acid with 30 s had a higher callus induction ratio than those not treated with concentrated sulfuric acid.Results also indicated that sodium hypochlorite was more efficient than mercury bichloride for Centipede grass callus induction. |
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| Keywords: | Centipedegrass Sterilization methods Callus induction |
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