| 油茶查尔酮合酶和异构酶基因的cDNA克隆 |
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| 引用本文： | 谭晓风,张党权,陈鸿鹏,谢禄山,王晓红,石明旺,胡芳名.油茶查尔酮合酶和异构酶基因的cDNA克隆[J].中南林业科技大学学报,2007,27(1):9-13. |
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| 作者姓名： | 谭晓风 张党权 陈鸿鹏 谢禄山 王晓红 石明旺 胡芳名 |
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| 作者单位： | 中南林业科技大学经济林育种与栽培国家林业局重点实验室 湖南长沙410004 |
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| 基金项目： | 国家自然科学基金;湖南省自然科学基金 |
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| 摘 要： | 查尔酮合酶和查尔酮异构酶是类黄酮代谢与色素苷代谢的关键酶.以油茶近成熟种子cDNA文库和EST文库为材料,通过分子克隆方法鉴定了1条查尔酮合酶基因全长cDNA和1条查尔酮异构酶全长cDNA.结果表明:油茶查尔酮合酶基因的cDNA含1479 bp,编码412个氨基酸,为目前最长的查尔酮合酶,与其它物种的查尔酮合酶具有极高的相似性,在进化上高度同源;油茶查尔酮异构酶基因的cDNA含899 bp,编码206个氨基酸,与茶的查尔酮异构酶基因高度同源,但与其它物种的相似性较低.
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| 关 键 词： | 经济林 油茶 查尔酮合酶 查尔酮异构酶 全长cDNA 序列分析 基因克隆 |
| 文章编号： | 1000-2502（2007）01-0009-05 |
| 收稿时间： | 2006-12-10 |
| 修稿时间： | 2006-12-10 |
Cloning of cDNAs from Chalcone Synthase and Chalcone Isomerase of Camellia oleifera |
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| TAN Xiao-feng,ZHANG Dang-quan,CHEN Hong-peng,XIE Lu-shan,WANG Xiao-hong,SHI Ming-wang,HU Fang-ming.Cloning of cDNAs from Chalcone Synthase and Chalcone Isomerase of Camellia oleifera[J].Journal of Central South Forestry University,2007,27(1):9-13. |
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| Authors: | TAN Xiao-feng ZHANG Dang-quan CHEN Hong-peng XIE Lu-shan WANG Xiao-hong SHI Ming-wang HU Fang-ming |
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| Institution: | The Key Lab of Non-Wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha 410004, Hunan, China |
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| Abstract: | Chalcone synthase(CHS) and chalcone isomerase(CHI) are the key enzymes controlling the metabolism of flavonoid and anthocyanin.In this paper,1 full-length cDNA of CHS and 1 full-length cDNA of CHI were separated from the cDNA library and EST library of Camellia oleifera by the method of molecular cloning.The cDNA of C.oleifera CHS contains 1 479 bp for 412 amino acids,which is so far the longest CHS and displays high evolution homology with those other species for very high similarity.The cDNA of C.oleifera CHI contains 899 bp for 206 amino acids,and displays high homology with that of Camellia sinensis,but relatively low similarity with the other species. |
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| Keywords: | non-timber forest Camellia oleifera chalcone synthase chalcone isomerase full-length cDNA sequence analysis gene cloning |
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