| 云南德宏地区含羞草根瘤菌表型数值分类研究 |
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| 引用本文： | 王易鹏,刘晓云,张斌,陈赞,郭小叶.云南德宏地区含羞草根瘤菌表型数值分类研究[J].安徽农业科学,2010,38(16):8590-8591,8691. |
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| 作者姓名： | 王易鹏 刘晓云 张斌 陈赞 郭小叶 |
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| 作者单位： | 1. 西南林学院保护生物学学院,西南地区生物多样性保育国家林业局重点实验室,云南昆明,650224;河北大学生命科学学院,河北省微生物多样性研究与应用实验室,河北保定,071002
2. 河北大学生命科学学院,河北省微生物多样性研究与应用实验室,河北保定,071002 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 对分离自云南德宏瑞丽、潞西等地的86株含羞草植物根瘤菌株,和归属于Agrobacterium、Escherichia、Sinorhizobium、Burkholderia、Cupriavidus等属的6株已知参比菌株,进行了抗生素抗性、温度生长范围、碳氮源利用等88个表型性状研究。结果表明,该地区含羞草植物根瘤菌菌株中有15%的菌株能耐受5%的NaCl;菌株SWF67010、SWF67017的抗性较强,能耐受300μg/ml的四环素和卡那霉素以及5%的NaCl,并在4℃的条件下生长。试验数据通过MVSP4.1软件分析,得到数值分类树状图,供试菌株在78%相似水平上分为3个表观群,其中,第Ⅰ群5株未知菌株未与参比菌株聚群,第Ⅱ群3株未知菌株与中华根瘤菌属聚群,第Ⅲ群72株未知菌株与伯克霍尔德菌属聚群。
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| 关 键 词： | 含羞草 根瘤菌 表型性状 多样性 |
Phenotype Diversities of Rhizobia Isolated from Mimosa pudica in Yunnan Dehou Area |
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| WANG Yi-peng et al.Phenotype Diversities of Rhizobia Isolated from Mimosa pudica in Yunnan Dehou Area[J].Journal of Anhui Agricultural Sciences,2010,38(16):8590-8591,8691. |
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| Authors: | WANG Yi-peng |
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| Institution: | WANG Yi-peng et al(College of Protect Biology,Southwest Forestry College,Key Laboratory of Biodiversity Conservation in Southwest China of State Forestry Administration,Kunming,Yunnan 650224) |
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| Abstract: | 86 strains of rhizobia were isolated from Mimosa pudica nodules collected in Dehong Ruili,Luxi of Yunnan Province and 6 known species of Agrobacterium,Escherichia,Sinorhizobium,Burkholderia,Cupriavidus,uses of antibiotic resistance,temperature use,nutrient utilization Etc.by performing numerical analysis of 88 phenotypic feature.The results showed that 15% of the strains could tolerate 5% NaCl.Strain SWF67010,SWF67017 were able to withstand the test 300 μg/ml tetracycline and kanamycin,5% NaCl and 4 ℃ under the conditions of growth.Through the MVSP software analysis,obtaining numerical taxonomy tree,it was found that tested strains were divided into three apparent groups at 78% similarity level.The first group of 5 unknown strains was not with the reference strains of clusters,the second groups of 3 unknown strains clustered with Sinorhizobium melitoti USDA1002T,the third group of 72 unknown strains were grouped with Burkholderia cluster. |
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| Keywords: | Mimosa pudica Rhizobia Phenotypic characters Diversity |
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