| Abstract: | Bull sperm was experimentally contaminated with Mycoplasma (M.) bovis, M. bovigenitalium, and Acholeplasma laidlawii (10(5) colony-forming units/ml sperm). Antibiotics were tested for their effectiveness under conditions of preparation of sperm for artificial insemination, primarily for their capability of killing all mycoplasma by the end of sperm equilibration (cooling to +4 degrees C within 5 h). Mycoplasma decontamination was achievable only by means of nourseothricin and lincospectin, in a concentration of 0.8 mg/ml sperm diluent which did not yet impair bull sperm vitality. |
