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| 7株猪圆环病毒2型ORF2基因的克隆及序列分析 | |
| 引用本文： | 程 伟,张 志,吴发兴,张燕霞,李晓成,陈德坤.7株猪圆环病毒2型ORF2基因的克隆及序列分析[J].西北农业学报,2011,20(5):25-30. |
| 作者姓名： | 程 伟 张 志 吴发兴 张燕霞 李晓成 陈德坤 |
| 作者单位： | 1. 西北农林科技大学,陕西杨凌,712100;中国动物卫生与流行病学中心,山东青岛,266032 2. 中国动物卫生与流行病学中心,山东青岛,266032 3. 西北农林科技大学,陕西杨凌,712100 |
| 基金项目： | 农业部动物疫情防制与监测项目 |
| 摘 要： | 根据GenBank中猪圆环病毒2型的ORF2序列,设计l对引物,运用PCR方法,对中国动物卫生与流行病学中心监测室从福建、湖南和辽宁3省分离的7个毒株进行ORF2基因扩增.将其克隆到pGEM-T easy载体,筛选阳性重组质粒进行测序.应用DNAstar软件将测序结果与国内外参考毒株进行比对,并对Cap蛋白的细胞表位、糖基化位点等进行分析.结果表明,HUN、HUN-2和LN-19分离株ORF2基因大小为702 bp,编码233个氨基酸;FJ-3、FJ-4、HUN-11和LN-3的ORF2基因大小为705 bp,编码234个氨基酸.Cap蛋白5个表位区A(47～63)、B(65～87)、C(113～139)、D(165～200)和E(C端最后4个氨基酸)均发生一定变异.同源性分析表明,24株序列同源性为90.5%～99.9%,FJ-3与美国株同源性最低(90.5%),HUN和AF055393、DQl80392同源性最高(99.9%);FJ-3和FJ-4为94.5%,HUN-11和HUN-2为94.3%,HUN和HUN-2为99.3%,HUN和HUN-11为95%,LN-3和LN-19为94.7%,表明各地区ORF2基因比较稳定,但也存在一定差异. |
| 关 键 词： | 猪圆环病毒2型 克隆 序列分析 |
Cloning and Sequence Analysis of ORF2 Gene of Seven PCV2 Strains |
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| CHENG Wei,ZHANG Zhi,WU Faxing,ZHANG Yanxi,LI Xiaocheng and CHEN Dekun.Cloning and Sequence Analysis of ORF2 Gene of Seven PCV2 Strains[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(5):25-30. | |
| Authors: | CHENG Wei ZHANG Zhi WU Faxing ZHANG Yanxi LI Xiaocheng and CHEN Dekun |
| Institution: | CHENG Wei1,2,ZHANG Zhi2,WU Faxing2,ZHANG Yanxia2,LI Xiaocheng2 and CHEN Dekun1(1.Northwest A&F University,Yangling Shaanxi 712100,China,2.China Animal Health and Epidemiology Center,Qingdao Shandong 266032,China) |
| Abstract: | In order to know the heredity disciplinarian of porcine circovirus(PCV) and pave the basement for preventing and controlling the disease.In this research,according to the published ORF2 gene sequence of PCV2,a pair of specific PCR primers were designed,and ORF2 genes was amplified from PCV2 strains isolated by China Animal Health and Epidemiology Center monitoring room from Fujian,Hunan and Liaoning.Then the PCR products were cloned into pGEM-T easy vector,and the recombinant plasmids were obtained and sequ... |
| Keywords: | PCV2 Cloning Sequence analysis |
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