| 除精浆提高冷冻精液品质的实验研究 |
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| 引用本文： | 姬云涛,屈长青,曹斌云.除精浆提高冷冻精液品质的实验研究[J].中国农学通报,2005,21(11):9-9. |
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| 作者姓名： | 姬云涛 屈长青 曹斌云 |
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| 作者单位： | （西北农林科技大学动物科技学院，陕西杨凌712100） |
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| 摘 要： | 采用离心洗涤法去除精浆后，对精液进行冷冻处理。发现：1、除精浆精子冻后的活率、Ⅰ类顶体率和精子畸形率与未除精浆组存在显著差异(P<0.05)；2、除精浆使稀释后精液精浆中GOT和LDH活性明显升高(P<0.05)。从平衡后到解冻后各组GOT和LDH活性都呈上升趋势。但除精浆试验组比未除精浆试验组上升缓慢；3、除精浆试验组精子的穿卵率、卵内平均精子数都显著高于未除精浆试验组(P<0.05)；4除精浆离心洗涤液用生理盐水和脱脂乳-卵柠液，两者之间无显著差异(P>0.05)。
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| 关 键 词： | 离心 除精浆 冷冻保存 精子品质 |
Study on Improving Frozen-thawed Sperm Quality |
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| Ji Yuntao,Qu Changqing,Cao Binyun.Study on Improving Frozen-thawed Sperm Quality[J].Chinese Agricultural Science Bulletin,2005,21(11):9-9. |
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| Authors: | Ji Yuntao Qu Changqing Cao Binyun |
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| Institution: | (College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100 ) |
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| Abstract: | The sperm being removed seminal plasma and not being removed seminal plasma by centrifugation were cryopreservated.1.The percentages of the frozen-thawed sperm of motility, normal acrosome and abnormal spermatozoa were significant difference between the tw |
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| Keywords: | Seminal plasmazz Frozen-thawed spermzz Centrifugationzz |
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