| 应用微卫星DNA标记分析荷斯坦母牛系谱可靠性及影响因素 |
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| 引用本文： | 初芹,张毅,孙东晓,俞英,王雅春,张沅.应用微卫星DNA标记分析荷斯坦母牛系谱可靠性及影响因素[J].畜牧兽医学报,2011,42(2). |
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| 作者姓名： | 初芹 张毅 孙东晓 俞英 王雅春 张沅 |
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| 作者单位： | 1. 中国农业大学动物科技学院,北京,100193;北京市农林科学院畜牧兽医研究所,北京,100097
2. 中国农业大学动物科技学院,北京,100193 |
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| 基金项目： | 国家科技支撑计划(2006BAD04A01;2006BAD04A13); 公益性行业科研专项(nyhyzx07-036-13); 国家奶牛产业技术体系资金; 北京市科技计划(D08060500070801); 863项目(2007AA10Z157); 转基因专项(2009ZX08009-156B) |
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| 摘 要： | 为了评估当前中国荷斯坦牛群体的系谱准确性,本研究选择15头种公牛及来自21个牛场的2 220头女儿牛,借助牛3号染色体(BTA3)上17个微卫星标记,对女儿-公牛标记基因型是否错配进行分析。结果,按照错配标记数大于等于3个为系谱错误的标准,本研究所涉及的母牛群中系谱错误率为16.62%。经对影响系谱错误的因素分析表明,场效应是主要的影响因素,不同场间系谱错误比例差异较大;由于公牛女儿在各牛场中分布不均匀,导致公牛效应也达到显著。研究结果提示在中国荷斯坦牛群体进行亲子鉴定是亟需和必要的。
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| 关 键 词： | 微卫星 亲子鉴定 系谱错误 中国荷斯坦牛 |
Pedigree Verification of Holstein Cows and Analysis of Factors Affecting Incorrect Paternity Based on Microsatellite Markers |
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| CHU Qin,ZHANG Yi,SUN Dong-xiao,YU Ying,WANG Ya-chun,ZHANG Yuan.Pedigree Verification of Holstein Cows and Analysis of Factors Affecting Incorrect Paternity Based on Microsatellite Markers[J].Acta Veterinaria et Zootechnica Sinica,2011,42(2). |
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| Authors: | CHU Qin ZHANG Yi SUN Dong-xiao YU Ying WANG Ya-chun ZHANG Yuan |
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| Institution: | CHU Qin1,2,ZHANG Yi1,SUN Dong-xiao1,YU Ying1,WANG Ya-chun1,ZHANG Yuan1 (1.College of Animal Science and Technology,China Agricultural University,Beijing 100193,China,2.Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agricultureand Forestry Sciences,Beijing 100097,China) |
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| Abstract: | To verify the pedigree data of Chinese Holstein cows,17 microsatellites on BTA3 were genotyped in 15 Chinese Holstein sires and their 2 220 daughters,which came from 21 herds in Beijing.The paternity with conflicts between the putative sire and daughter in at least 3 markers was considered to be erroneous.The paternity error rate was 16.62% in the Chinese Holstein population.Factors affecting incorrect paternity were further analyzed and results showed that the paternity error rate varied extremely signific... |
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| Keywords: | microsatellite paternity testing paternity error Chinese Holstein |
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