| 苹果属部分观赏品种与中国野生种的亲缘关系 |
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| 引用本文： | 张宁,沈红香,高遐虹,姚允聪,王洋,冯永庆.苹果属部分观赏品种与中国野生种的亲缘关系[J].园艺学报,2007,34(5):1227-1234. |
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| 作者姓名： | 张宁 沈红香 高遐虹 姚允聪 王洋 冯永庆 |
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| 作者单位： | (北京农学院植物科学技术系,北京102206） |
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| 基金项目： | 北京市科技新星计划人才培养项目 |
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| 摘 要： | 采用AFLP分子标记技术对47种苹果属植物进行遗传多样性和亲缘关系分析，其中24个为国外引进的观赏海棠品种。结果表明，筛选出的10对分辨率强的引物共扩增出589条带，其中多态性带534条，多态位点百分率达90.66%。观赏海棠品种在DNA分子水平上表现出极为丰富的遗传多样性。基于Jaccard系数对AFLP扩增结果进行UPGMA (Unweighted Pair Group Method with Arithmetic Mean)聚类，以相似系数0.60为标准，将供试材料分为8组，以相似系数0.59为标准，将观赏海棠品种分为4组。分析认为，供试的24个观赏海棠品种中有22个品种与我国原产的苹果属植物亲缘关系较近。
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| 关 键 词： | 观赏海棠 亲缘关系 遗传多样性 AFLP |
| 文章编号： | 0513-353X（2007）05-1227-08 |
| 修稿时间： | 2007-02-16 |
Phylogenetic Relationship Between Ornamental and Wild Species of Malus in China |
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| ZHANG Ning,SHEN Hong-xiang,GAO Xia-hong,YAO Yun-cong,WANG Yang,FENG Yong-qing.Phylogenetic Relationship Between Ornamental and Wild Species of Malus in China[J].Acta Horticulturae Sinica,2007,34(5):1227-1234. |
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| Authors: | ZHANG Ning SHEN Hong-xiang GAO Xia-hong YAO Yun-cong WANG Yang FENG Yong-qing |
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| Institution: | （Department of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China） |
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| Abstract: | In this study, 24 crabapple cultivars and 23 wild species of Malus were analyzed using AFLP (Amplified Fragment Length Polymorphism)molecular marker in order to test the genetic diversity and rela- tionship of the materials. The results showed that a total of 589 informative AFLP fragments were generated with 10 EcoR I-Mse I primer combinations, of which 534 ones were polymorphic and the polymorphism rate was 90. 66%. Rich genetic diversity of crabapple germplasm resources was detected at DNA level. UPGMA (Unweighted Pair-Group Method with Arithmetic Mean)cluster analysis based on Jaccard coefficient indicated that the materials used in the study could be divided into 8 groups, crabapple cultivars were divided into 4 groups. In the present study, 22 of 24 cultivars tested were closely related with species of Malus in China. |
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| Keywords: | AFLP |
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