|
|||||
|
|
| HPLC法测定大青叶中靛玉红的含量 | |
| 引用本文： | 王虹霞,梁剑平,李雪虎,陆锡宏,雷宏东.HPLC法测定大青叶中靛玉红的含量[J].湖南农业科学,2012(3):87-89. |
| 作者姓名： | 王虹霞 梁剑平 李雪虎 陆锡宏 雷宏东 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃兰州,730070 2. 中国农业科学院兰州畜牧与兽药研究所,甘肃兰州,730050 3. 中国科学院近代物理研究所,甘肃兰州,730000 |
| 基金项目： | 甘肃省重大科技专项(092G K D A0033);国家青年基金项目 |
| 摘 要： | 以HPLC法作为大青叶中靛玉红含量的检测方法,探讨了不同pH值和不同萃取溶剂对靛玉红提取的影响,确立了大青叶中靛玉红含量的测定方法。结果表明:在试验条件下,靛玉红在1.5～24μg/mL范围内与峰面积呈良好的线性关系(r=0.999 9),精密度RSD=0.64%,加样回收率为97.33%,重现性RSD=0.54%。HPLC法简单可行,准确性好,重现性高,是大青叶中靛玉红含量测定的有效方法。 |
| 关 键 词： | 大青叶 靛玉红 HPLC |
Measurement of Indirubin Content in Daqingye by HPLC |
|
| WANG Hong-xia , LIANG Jian-ping , LI Xue-hu , LU Xi-hong , LEI Hong-dong.Measurement of Indirubin Content in Daqingye by HPLC[J].Hunan Agricultural Sciences,2012(3):87-89. | |
| Authors: | WANG Hong-xia LIANG Jian-ping LI Xue-hu LU Xi-hong LEI Hong-dong |
| Institution: | 1(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,PRC; 2.Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,Chinese Academy of Agricultural Sciences, Gansu 730050,PRC;3.Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,PRC) |
| Abstract: | To establish a method for measuring the content of indirubin in Daqingye,the HPLC method was adopted to discuss the impacts of different pH values and different extraction solvents on extraction of indirubin from Daqingye.The results indicated that under the experimental conditions,indirubin showed a good linear correlation(r=0.999 9) with peak area within the range of 1.5~24 μg/mL,precision RSD=0.64%,the average recovery ratio was 97.33%,and the repeatability RSD=0.54%.Therefore,the HPLC method is a simple and feasible method with high accuracy and good reproducibility,which is an effective method for measurement of indirubin content in Daqingye. |
| Keywords: | Daqingye indirubin HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |