| RAPD标记在日本兵库县20个酒米品种类群划分中的应用 |
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| 引用本文： | 李余良,吉田晋弥.RAPD标记在日本兵库县20个酒米品种类群划分中的应用[J].华南农业大学学报,2000,21(4):4-6. |
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| 作者姓名： | 李余良 吉田晋弥 |
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| 作者单位： | [1]广东省农科院作物研究所,广东广州 [2]日本国兵库县立中央农业技术中心,广东广州 |
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| 基金项目： | 日本兵库县农业尖端技术项目 |
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| 摘 要： | 应用ＲＡＰＤ分子标记对日本兵库县２０个酒米品种的类群进行了研究。筛选出对不同基因型酒米品种ＤＮＡ扩增具有较好多态性的引物１１个，它们分别是Ａ０２、Ａ０８、Ｂ１８、Ｃ１５、Ｄ０３、Ｄ０８、Ｇ０５、Ｍ１１、Ｑ１６、Ｓ１３和Ｔ０６。根据这些随机引物对不同品种总ＤＮＡ具有特性性的扩增谱带，建立不同品种的ＲＡＰＤ多态性标准模式图，可以快速鉴别品种和进行纯度分析；依据扩增谱带建立０、１型数据，计算２０个酒米品
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| 关 键 词： | RAPD分子标记 酒米 类群划分 品种 纯度鉴定 |
The Application of RAPD Technology to Heredity Grouping of 20 Rice Varieties of Hyogo of Japan |
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| LI Yu liang,Yoshida Sinya.The Application of RAPD Technology to Heredity Grouping of 20 Rice Varieties of Hyogo of Japan[J].Journal of South China Agricultural University,2000,21(4):4-6. |
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| Authors: | LI Yu liang Yoshida Sinya |
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| Institution: | LI Yu liang 1,Yoshida Sinya 2 |
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| Abstract: | Heredity groups were classified by cluster analysis with RAPD technology based on 20 rice varieties of hyogo prefecture of Japan. 11 primers, A 02 , A 08 , B 18 , C 15 , D 03 , D 08 , G 05 , M 11 , Q 16 , S 13 and T 06 , selected from 220 candidate primers act as polymorphic DNA (RAPD) makers for 20 rice. The maps of RAPD standard polymorphic DNA in different varieties can be established by their specific characteristic bands. Thus, the cultivars and their purity could be identified and analysed quickly. In addition, Genetic distance among 20 rice varieties was calculated and clustered with RAPD makers. The results showed that 20 rice varieties could be classified into four groups. The four groups classified by cluster analysis with RAPD technology is consistent with the expected grouping based on available pedigree. The current study indicated that it was practical to group rice with RAPD technology. |
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| Keywords: | RAPD maker rice varieties group and cluster classified |
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