| 促进因子对阿舒假囊酵母合成核黄素的影响 |
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| 引用本文： | 刘志文,黄林,孔令宝,程新,涂晓嵘,李昆太.促进因子对阿舒假囊酵母合成核黄素的影响[J].江西农业大学学报,2010,32(2). |
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| 作者姓名： | 刘志文 黄林 孔令宝 程新 涂晓嵘 李昆太 |
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| 作者单位： | 1. 江西农业大学,生物科学与工程学院,江西,南昌,330045
2. 江西农业大学,生物科学与工程学院,江西,南昌,330045;南昌市发酵应用技术重点实验室,江西,南昌,330045 |
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| 基金项目： | 江西农业大学博士科研启动基金资助项目(08-2064) |
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| 摘 要： | 研究在发酵培养基中添加不同促进因子(玉米浆、酵母膏和豆油)对阿舒假囊酵母合成核黄素的影响。试验结果表明:添加10g/L玉米浆是最促进阿舒假囊酵母菌体生长和核黄素合成的最适质量浓度;40g/L酵母膏分别在0h补加20g/L,24h和48h各补加10g/L,其核黄素产量达到1587.37mg/L,比在0h1次添加40g/L酵母膏的产量提高了21.07%;在发酵培养基中添加2～5g/L豆油,对核黄素的合成均有促进作用。
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| 关 键 词： | 阿舒假囊酵母 核黄素 生物合成 促进因子 |
Effects of Stimulators on Riboflavin Biosynthesis by Eremothecium ashbyii |
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| LIU Zhi-wei,HUANG Lin,KONG Ling-bao,CHENG Xin,TU Xiao-rong,LI Kun-tai.Effects of Stimulators on Riboflavin Biosynthesis by Eremothecium ashbyii[J].Acta Agriculturae Universitis Jiangxiensis,2010,32(2). |
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| Authors: | LIU Zhi-wei HUANG Lin KONG Ling-bao CHENG Xin TU Xiao-rong LI Kun-tai |
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| Institution: | 1.College of Biological Science and Technology/a>;JAU/a>;Nanchang 330045/a>;China/a>;2.Nanchang Key Laboratory of Applied Fermentation Technology/a>;China |
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| Abstract: | In order to improve the productivity of riboflavin by Eremothecium ashbyii in shake-flask cultivation,the effects of various stimulators (corn steep liquor,yeast extract and bean oil) on riboflavin biosynthesis were investigated.The results showed that 10 g/L of corn steep liquor was optimal for cell growth and riboflavin production.When a total of 40 g/L of yeast extract was added to the fermentation broth at 0 h (20 g/L),24 h (10 g/L) and 48 h (10 g/L),respectively,a maximum riboflavin production (1 587.3... |
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| Keywords: | Eremothecium ashbyii riboflavin biosynthesis stimulators |
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