Protective Effects of Interleukin-4 on Tissue Destruction and Morphological Changes of Bovine Nasal Chondrocytes in vitro |
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| Authors: | Maryam Yadegari Mahmoud Orazizadeh Mahmoud Hashemitabar Ali Khodadadi |
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| Institution: | 1.Dept. of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Faculty of Medicine, Yazd, Iran;;2. Dept. of Anatomical Sciences, Cellular and Molecular Research Center (CMRC), Ahvaz Jundishapur University of Medical Sciences, Faculty of Medicine, Ahvaz, Iran;;3.Dept. of Immunology, Ahvaz Jundishapur University of Medical Sciences, Faculty of Medicine, Ahvaz, Iran |
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| Abstract: | Background: Previous studies have shown that some cytokines have protective effects on cartilage in joint diseases. In the current study, effects of IL-4 against morphological changes and tissue degradation induced by IL-1α on bovine nasal cartilage (BNC) explants were investigated. Methods: Fresh BNC samples were prepared from a slaughterhouse under sterile conditions. BNC explants culture was treated with both IL-lα (10 ng/ml) and IL-4 (50 ng/ml) at the same time for 28 days. The morphological characteristics of explants were assessed by using histology techniques and invert microscopy. Matrix metalloproteinase-1 (MMP-1) production was assessed within different days by using Western blotting. Results: IL-lα induced prominent cartilage morphology degradation. The pro and active form of MMP-1 band substantially increased at day 21 of culture. In the presence of both IL-lα and IL-4, chondrocytes preserved their ordinary normal phenotype with intact extracellular matrix. In addition, a significant reduction in pro-MMP-1and inhibition of active MMP-1 was seen. Conclusion: In conclusion, IL-4 could be regarded as a potential candidate in cartilage protecting against the degradation changes of IL-lα. It seems that the preservation effect of IL-4 is associated with significant reduction of MMP-1. Key Words: Chondrocyte, Interleukin-1α, Interleukin-4, Matrix metalloproteinase-1, Bovine nasal cartilage |
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