| 球根海棠离体培养的形态发生研究 |
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| 引用本文： | 刘福平,苏明华,章宁,林清洪.球根海棠离体培养的形态发生研究[J].园艺学报,2004,31(5):694-696. |
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| 作者姓名： | 刘福平 苏明华 章宁 林清洪 |
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| 作者单位： | (福建省亚热带植物研究所, 厦门361006) |
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| 基金项目： | 福建省重大科技项目 (99-I-1)，福建省自然科学基金项目 (C0 410 0 40 ) |
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| 摘 要： | 球根海棠外植体可在同一条件下同时诱导不定芽和胚状体, 经间接途径和直接途径的体细胞胚胎发生频率均很高, 体细胞胚为单细胞起源或多细胞起源。以再生嫩叶为继代中间体, 在较高温度(28 ℃) 下诱导, 可建立高效扩繁体系。纵切芽丛继代形成大量愈伤组织, 愈伤组织继代半年后几乎完全丧失分化能力, 发生激素驯化作用, 而且愈伤组织细胞染色体数目已发生广泛变异。
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| 关 键 词： | 球根海棠 体细胞胚胎发生 再生体系 继代愈伤组织 |
| 文章编号： | 0513-353X(2004)05-0694-03 |
| 收稿时间： | 2003-12-17 |
| 修稿时间： | 2004-7-23 |
In Vitro Morphogenesis of Begonia tuberhybrida |
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| Liu Fuping,Su Minghua,Zhang Ning,and Lin Qinghong.In Vitro Morphogenesis of Begonia tuberhybrida[J].Acta Horticulturae Sinica,2004,31(5):694-696. |
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| Authors: | Liu Fuping Su Minghua Zhang Ning and Lin Qinghong |
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| Institution: | ( Fujian Institute of Subtropical Botany , Xiamen 361006 , China) |
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| Abstract: | Organogenesis and somatic embryogenesis of Begonia tuberhybrida could carry out on the same culture conditions. Somatic embryos occurred from explant directly or indirectly and originated f rom single-cell or multi-cell. At higher induce temperature (28 ℃) , subculturing regenerated young leaf could establish high efficient regenerate system. Through subculturing cluster of buds , a large amount of callus formed , and the callus subcultured for half a year lost almostly differential ability. Observation showed callus was habituated by growth substance and there were wide-ranging variation in the number of chromosome in cells of the callus. |
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| Keywords: | Begonia tuberhybrida Somatic embryogenesis Regenerate system Subculture callus$$$$ |
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