| 2拷贝SLT-ⅡvB在猪霍乱沙门氏菌C500crp~-/asd~-缺失株中的表达及小鼠免疫试验 |
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| 引用本文： | 徐引弟,王治方,朱文豪,郭成留.2拷贝SLT-ⅡvB在猪霍乱沙门氏菌C500crp~-/asd~-缺失株中的表达及小鼠免疫试验[J].中国畜牧兽医,2010,37(7):70-73. |
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| 作者姓名： | 徐引弟 王治方 朱文豪 郭成留 |
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| 作者单位： | (河南省农业科学院畜牧兽医研究所，郑州 450002) |
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| 基金项目： | 河南省科技攻关计划项目，河南省农科院高技术产品孵化项目 |
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| 摘 要： | 为研制仔猪副伤寒—猪水肿病新型二联疫苗,从pMDSLT-Ⅱ中扩增去信号肽后的SLT-ⅡvB基因,将2拷贝SLT-ⅡvB与pYA3493连接。阳性质粒pYA2B电转化C500crp-/asd-缺失株,进行SDS-PAGE电泳和Western blotting,重组菌C500crp-/asd-(pYA2B)口服免疫小鼠,腹腔攻10 LD50的猪霍乱沙门氏菌强毒C78-1和2 LD50的猪水肿大肠杆菌强毒ED3株,计算攻毒保护率。结果显示,2 SLT-ⅡvB在C500crp-/asd-缺失株中获得了表达。C500crp-/asd-(pYA2B)免疫组对ED3的攻击能提供60%保护,而对照组不能提供任何保护。2组对C78-1攻击均提供100%保护,为研制仔猪副伤寒—猪水肿病二联疫苗及进一步提高免疫效果提供依据。
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| 关 键 词： | 猪霍乱沙门氏菌C500crp-/asd-缺失株 宿主载体平衡致死系统 2拷贝SLT-ⅡvB 基因表达 免疫反应 |
Expression of 2 Copies SLT-II vB Gene in C500crp-/asd-Mutant of Salmonella choleraesuis and Immune Test on Mice |
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| XU Yin-di,WANG Zhi-fang,ZHU Wen-hao,GUO Cheng-liu.Expression of 2 Copies SLT-II vB Gene in C500crp-/asd-Mutant of Salmonella choleraesuis and Immune Test on Mice[J].China Animal Husbandry & Veterinary Medicine,2010,37(7):70-73. |
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| Authors: | XU Yin-di WANG Zhi-fang ZHU Wen-hao GUO Cheng-liu |
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| Institution: | （Institute of Animal Husbandry and Veterinary Medicine, Henan Academy ofAgricultural Sciences, Zhengzhou 450002, China） |
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| Abstract: | In order to develop novel piglet’s Paratyphoid-ED bivalent vaccine, SLT-ⅡvB gene without signal peptide was amplified from pMDSLT-Ⅱ and subcloned into pYA3493. pYA2B was electroporated into C500crp-/asd- mutant. SDS-PAGE and Western blotting were done. Mice were i.p. immunized with C500crp-/asd-(pYA2B) and C500crp-/asd-(pYA3493) and i.p. challenged with 2 LD50 ED3 and 10 LD50 C78-1. 2 SLT-ⅡvB was expressed in C500crp-/asd- mutant. The C500crp-/asd-(pYA2B) immunized group was 60% protected mice against ED3 challenging and control groups couldn’t protect mice. All immunized groups induced 100% protected mice against C78-1 challenging. These results provided base for developing piglet’s Paratyphoid-ED bivalent vaccine and further improving of immunity effect. |
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| Keywords: | S choleraesuis C500crp-/asd- mutant balanced lethal host-vector system 2 copies SLT-ⅡvB gene expression immune test |
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