| 氯虫苯甲酰胺亚致死剂量对棉铃虫生长发育和解毒酶活性的影响 |
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| 引用本文： | 欧善生,梁沛,宋敦伦,史雪岩,高希武.氯虫苯甲酰胺亚致死剂量对棉铃虫生长发育和解毒酶活性的影响[J].植物保护,2012,38(4):1-8. |
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| 作者姓名： | 欧善生 梁沛 宋敦伦 史雪岩 高希武 |
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| 作者单位： | 1. 广西农业职业技术学院,南宁530007;中国农业大学农学与生物技术学院,北京100193
2. 中国农业大学农学与生物技术学院,北京,100193 |
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| 基金项目： | 公益性行业(农业)科研专项经费项目 |
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| 摘 要： | 在室内采用培养基混药法测定氯虫苯甲酰胺亚致死剂量(LC10、LC25和LC50)对棉铃虫生长发育和体内3种解毒酶系的影响。结果表明:各剂量处理3龄幼虫48h后饲养,各处理组当代(F0)3～6龄幼虫历期均比对照组延长0.2～3.8d,但成虫平均历期比对照组缩短0.8～2.5d,子代(F1)1～6龄幼虫历期均比对照组延长0.2～2.6d,成虫平均历期比对照组缩短0.1～0.5d;各剂量处理后,各处理组F0代3～6龄幼虫和蛹的体重、F1代4～6龄幼虫和蛹的体重均比对照组减轻,且剂量越大,体重减轻越显著;各处理组F0代种群趋势指数分别比对照组下降了16.27%、58.51%和71.17%,F1代分别下降了13.66%、52.61%和66.63%;各处理组F0代3～6龄幼虫体内的羧酸酯酶(CarE)和细胞色素P450O-脱乙基酶(P450-ECOD)比活力均高于对照组,其中6龄幼虫这两种酶比活力分别比对照组提高了5%～16%和23%～66%,但各处理组谷胱甘肽硫转移酶(GSTs)比活力均低于对照组,其中6龄幼虫GSTs比活力分别比对照组降低了16.23%、58.51%和71.17%。表明在LC10～LC50剂量范围内,氯虫苯甲酰胺处理棉铃虫3龄幼虫的亚致死剂量越高,其生长发育受抑制越显著,P450-ECOD和CarE比活力上升越高,GSTs比活力下降也越大。
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| 关 键 词： | 氯虫苯甲酰胺 棉铃虫 亚致死剂量 生长发育 解毒酶 |
Effects of sublethal dosage of chlorantraniliprole on development and detoxifying enzymes activity of Helicoverpa armigera |
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| Ou Shansheng,Liang Pei,Song Dunlun,Shi Xueyan,Gao Xiwu.Effects of sublethal dosage of chlorantraniliprole on development and detoxifying enzymes activity of Helicoverpa armigera[J].Plant Protection,2012,38(4):1-8. |
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| Authors: | Ou Shansheng Liang Pei Song Dunlun Shi Xueyan Gao Xiwu |
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| Institution: | 1.Guangxi Vocational Technical College of Agriculture,Nanning 530007,China; 2.College of Agriculture and Biotechnology,China Agricultural University,Beijing 100193,China) |
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| Abstract: | This research,by blending the targeted chemical into culture medium,aims to study the effects of three sub-lethal doses(i.e.LC10,LC25,and LC50)of chlorantraniliprole on the development and the activities of three detoxifying enzymes of Helicoverpa armigera Hübner.The results showed that,after treatment with three different sub-lethal doses to the third-instar larvae for 48 hours,the development durations of the treated third-to sixth-instar larvae(F0) were 0.2-3.8 d longer than that of the control,and the average development durations of the adults were 0.8-2.5 d shorter than that of the control;the durations of 1st-6th-instar larvae of the offspring(F1) were lengthened by 0.2-2.6 d compared with the control;the average duration of the adult was shortened by 0.1-0.5 d compared with the control.Compared with the control,the weights of the third-instar to sixth-instar larvae and their pupae(F0) and the fourth-to sixth-instar larvae and the pupae(F1) were decreased,which was correlated with the dose negatively;the population trend indexes of the groups treated with LC10,LC25,and LC50 decreased by 16.27%,58.51% and 71.17% in F0 and 13.66%,52.61% and 66.63% in F1,respectively;by comparison with the control,the activities of CarE and P450-ECOD of the third-instar to sixth-instar larvae(F0) were significantly higher,especially in the sixth-instar larvae,the activities of the two detoxification enzymes were strengthened by 5%-16% and 23%-66%,respectively.In contrast,the activities of GSTs of the groups treated with three different sub-lethal doses were lower than that of the control,and in the sixth-instar larvae,the activity decreased by 16.23%,58.51% and 71.17%,respectively.This suggest that,within the range from LC10 to LC50,the higher the sub-lethal doses of chlorantraniliprole treating the third-instar H.armigera larvae,the more significant the suppression effect on the development is,and the higher the activities of CarE and P450-ECOD,the lower the activity of GSTs. |
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| Keywords: | chlorantraniliprole Helicoverpa armigera sublethal dosage growth detoxifying enzyme |
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