| 小菜蛾鱼尼丁受体基因克隆及序列分析 |
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| 引用本文： | 孙丽娜,芮昌辉,袁会珠.小菜蛾鱼尼丁受体基因克隆及序列分析[J].植物保护,2012,38(4):118-123. |
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| 作者姓名： | 孙丽娜 芮昌辉 袁会珠 |
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| 作者单位： | 中国农业科学院植物保护研究所,农业部作物有害生物综合治理综合性重点实验室,北京 100193 |
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| 基金项目： | 国家重点基础研究发展计划(“973”计划)项目 |
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| 摘 要： | 利用RT-PCR和RACE技术克隆了小菜蛾(Plutella xylostella)鱼尼丁受体(Px-RyR)基因,其核苷酸序列全长15 748bp,5′非编码区267bp,3′端非编码区109bp,开放阅读区全长为15 372bp(GenBank登录号:JF927788),编码5 123个氨基酸残基。估测其蛋白分子量为579.39ku,等电点为5.45。该基因编码氨基酸序列和其他鳞翅目昆虫RyR氨基酸序列比对相似性较高(92%),与哺乳动物3种亚型RyRs的相似性为45%～47%。此外,二级结构预测,其C-末端存在6个跨膜区域;且Px-RyR中存在一个出现4次重复,长度为89～95个氨基酸的RyR结构域,平均相似性为33%。
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| 关 键 词： | 小菜蛾 鱼尼丁受体 基因克隆 |
Molecular cloning and sequence analysis of ryanodine receptor gene in the diamondback moth, Plutella xylos tella |
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| Sun Lin,Rui Changhui,Yuan Huizhu.Molecular cloning and sequence analysis of ryanodine receptor gene in the diamondback moth, Plutella xylos tella[J].Plant Protection,2012,38(4):118-123. |
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| Authors: | Sun Lin Rui Changhui Yuan Huizhu |
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| Institution: | (Laboratory of Integrated Pest Management in Crops,Ministry of Agriculture;Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | The full-length cDNA of the ryanodine receptor from the lepidopteran pest Plutella xylostella(Px-RyR) was 15 748 bp,containing a 5′-UTR of 267 bp,a 3′-UTR of 109 bp and a full open reading frame(ORF) of 15 372 bp(GenBank accession No.JF927 788).The amino acid sequence deduced from the sequence was 5 123 amino acids.The calculated molecular weight for Px-RyR was 579.39 ku,and its isoelectric point was 5.45.The deduced amino acids sequence of Px-RyR showed a high level of similarity with the RyRs sequences from other Lepidoptera insects,such as the tobacco budworm Heliothis virescens(92%) and silkworm Bombyx mori(92%).It shared 45%-47% similarity with the vertebrate isoforms.In addition,there were six essential transmembrane(TM) segments,predicted from the secondary structure of the deduced amino acid sequence at C-terminal of Px-RyR.A repeated sequence motif of 89-95 amino acids was observed,sharing 33% similarity on average and occurring four times. |
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| Keywords: | Plutella xylostella ryanodine receptor molecular cloning |
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