| 大肠埃希菌耐药谱型、基因型和生物膜表型关系研究 |
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| 引用本文： | 陈朝喜,朱恒乾,廖晓萍,刘雅红.大肠埃希菌耐药谱型、基因型和生物膜表型关系研究[J].动物医学进展,2011,32(4):59-62. |
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| 作者姓名： | 陈朝喜 朱恒乾 廖晓萍 刘雅红 |
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| 作者单位： | 1. 华南农业大学兽医学院兽医药理与毒理学教研室,广东广州510642;西南民族大学生命科学与技术学院兽医药理学教研室,四川成都610041
2. 华南农业大学兽医学院兽医药理与毒理学教研室,广东广州,510642 |
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| 基金项目： | 国家自然科学基金-广东省联合基金 |
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| 摘 要： | 以ATCC25922为研究对象,采用改良结晶紫染色,快速银染法和扫描电镜技术对其生物被膜进行定量和定性研究,建立大肠埃希菌生物被膜体外模型,利用优化的模型条件对249株临床分离株进行验证.采用琼脂平板计数法绘制大肠埃希菌浮游菌和被膜菌生长曲线,比较其生长特性的异同.对不同成膜能力的大肠埃希菌临床分离株进行色氨酸定量试验...
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| 关 键 词： | 耐药谱型 生物被膜表型 基因型 肠杆菌基因间重复一致序列聚合酶链反应 |
Study on Relationship between Drug Resistance Pattern, Biofilm Phenotype and Genetic Phenotype in E.coli |
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| CHEN Chao-xi,ZHU Heng-qian,LIAO Xiao-ping,LIU Ya-hong.Study on Relationship between Drug Resistance Pattern, Biofilm Phenotype and Genetic Phenotype in E.coli[J].Progress In Veterinary Medicine,2011,32(4):59-62. |
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| Authors: | CHEN Chao-xi ZHU Heng-qian LIAO Xiao-ping LIU Ya-hong |
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| Institution: | 1(1.College of Veterinary Medcine,South China Agricultural University,Guangzhou,Guangdong,510642,China;2.College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan,610041,China) |
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| Abstract: | Broth microdilution method was used for antimicrobial agent susceptibility testing of 249 clinical E.coli isolates to 13 antibiotics,such as Enrofloxacin,Florfenicol and Ceftiofur and the data was statisticized by WHONET 5.4 software,and the relationship between drug resistance pattern and biofilm phenotype was analyzed.Meanwhile,ERIC-PCR(Enterobacterial repetitive intergenic consensus-PCR) technique was used for genetic fingerprint of the 64 clinical isolates to elucidate the relationship between biofilm-forming ability and genetic phenotype and provide theoretical basis for molecular genetic mechanisms and molecular epidemiology of E.coli biofilm formation. |
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| Keywords: | drug resistance pattern biofilm phenotype genetic phenotype ERIC-PCR |
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