Characteristics of Collagen from Rohu (Labeo rohita) Skin |
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| Authors: | Wanticha Savedboworn Soottawat Benjakul Sittichoke Sinthusamran Hideki Kishimura |
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| Institution: | 1. Department of Agro-Industry Technology and Management, Faculty of Agro-Industry, King Mongkut’s University of Technology North Bangkok, Muang, Thailand;2. Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Thailand;3. Laboratory of Marine Products and Food Science, Research Faculty of Fisheries Sciences, Hokkaido University, Hokkaido, Japan |
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| Abstract: | Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were isolated from rohu skin with the yield of 64.2 and 6.8% (dry weight basis), respectively. Both collagens had glycine as the major amino acid with imino acid content of 196–202 residues/1,000 residues and were characterized as type I collagen with molecular composition of (α1)2α2-heterotrimer. Fourier transform infrared spectra of both collagens were similar, with no shift in wavenumber of all amide bands. The Tmax value of ASC and PSC was 36.40 and 35.48°C, respectively. The zero surface net charge of ASC and PSC was found at pH 5.9 and 5.3, respectively. |
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| Keywords: | Collagen ASC PSC rohu Labeo rohita type I collagen |
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