| 禽致病性大肠杆菌中四种抗氨基糖苷类药物耐药基因的分子流行病学调查 |
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| 引用本文： | 金文杰,秦爱建,郑志明,黄训良,邵红霞,刘岳龙,张永志.禽致病性大肠杆菌中四种抗氨基糖苷类药物耐药基因的分子流行病学调查[J].中国预防兽医学报,2007,29(5):401-404. |
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| 作者姓名： | 金文杰 秦爱建 郑志明 黄训良 邵红霞 刘岳龙 张永志 |
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| 作者单位： | 扬州大学,江苏省动物预防医学重点实验室,江苏,扬州,225009 |
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| 基金项目： | 国家高技术研究发展计划(863计划);江苏省自然科学基金;江苏省教育厅资助项目 |
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| 摘 要： | 氨基糖苷类药物曾经是临床最为常用而有效的抗生素,长期应用与不合理使用使得该药物效果不尽理想。本研究参照GenBank中耐药基因相关序列,设计引物,结果从禽源大肠杆菌基因组中扩增出4种抗氨基糖苷类药物基因aadA1、strA、strB、aph(3′),经pGEX-T-easy和pET32a载体克隆和序列分析,证明扩增获得的基因序列与GenBank中参考序列同源性达到97%以上。对分离保存的216株禽致病性大肠杆菌进行氨基糖苷类药物耐药基因的分子流行病学检测,结果表明:aadA1阳性率高达49.1%,strA和strB的阳性率分别为56%和65.7%,aph(3′)的阳性率为16.2%;近三分之二的被检菌株携带有2种以上氨基糖苷类药物耐药基因。药敏试验结果显示所有被检菌株对链霉素耐药率为68.9%,而对阿米卡星的耐药率为38.9%。本研究结果说明禽类细菌的耐药性与相关耐药基因的检出率基本呈正相关,临床日益严重的耐药现象与耐药基因的普遍存在有着很大的关系,提示控制禽源耐药细菌对人类健康与卫生安全有重要意义。
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| 关 键 词： | 氨基糖苷类药物耐药基因 禽致病性大肠杆菌 流行病学 |
| 文章编号： | 1008-0589（2007）05-0401-04 |
| 收稿时间： | 2006-06-16 |
| 修稿时间： | 2006年6月16日 |
Molecular epidemiology of aminoglycoside resistant genes in avian pathogenicity E.coli |
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| JIN Wen-jie,QIN Ai-jian,ZHENG Zhi-ming,HUANG Xun-liang,SHAO Hong-xia,LIU Yue-long,ZHANG Yong-zhi.Molecular epidemiology of aminoglycoside resistant genes in avian pathogenicity E.coli[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(5):401-404. |
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| Authors: | JIN Wen-jie QIN Ai-jian ZHENG Zhi-ming HUANG Xun-liang SHAO Hong-xia LIU Yue-long ZHANG Yong-zhi |
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| Abstract: | Aminoglycoside as an antibiotics has become less effective over time.In order to understand the mechanism,PCR primers were designed according to the sequences of resistant gene aadAl,strA,strB,aph(3')published in GenBank.PCR products of 529 bp and 806 bp,837 bp,816 bp were obtained for the respective genes.Sequence analysis showed that these PCR fragments shared more than 97% sequence homology with the reference sequences published in GenBank.A total of 216 avain E.coli isolates were tested for resistant genes by PCR and 49.1%,56%,65.7% and 16.2% of tested strains were positive for aadAl gene and for strA,strB and aph(3'),respectively.In addition,more than two aminoglycoside resistant genes existed in nearly two thirds of the tested strains.Drug sensitive test showed that 68.9% of all tested isolates were resistant to streptomycin and 38.9% were resistant to amikacin.These results indicated that more and more serious drug resistance was related to the prevalence of drug resistant genes.Therefore control the resistant gene shift to human from avian is important for public health. |
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| Keywords: | aminoglycoside resistant genes avian pathgenicity E coli(APEC) epidemiology |
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