| 芜菁花叶病毒外壳蛋白基因的克隆与序列分析 |
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| 引用本文： | 庄木,王晓武,李艳双,刘玉梅,张扬勇,方智远.芜菁花叶病毒外壳蛋白基因的克隆与序列分析[J].中国蔬菜,2006,1(3):6-8. |
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| 作者姓名： | 庄木 王晓武 李艳双 刘玉梅 张扬勇 方智远 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所 |
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| 摘 要： | 将来自北京地区甘蓝和芥菜上的TuMV外壳蛋白基因片段分别克隆到pMD18-T载体中,测序并进行序列分析。结果表明:两者CP基因序列长度均为867个核苷酸,编码288个氨基酸,它们的核苷酸同源性为97·2%,氨基酸同源性为97.6%;两者与GenBank中的部分CP基因序列的核苷酸同源性在87.8%~97.8%之间,氨基酸同源性在91.7%~99.0%之间;分子进化树分析两者可归属World-B组。
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| 关 键 词： | 芜菁花叶病毒 外壳蛋白基因 序列分析 |
| 收稿时间： | 2005-10-14 |
| 修稿时间： | 2005年10月14 |
Cloning and Sequence Analysis of Turnip Mosaic Virus Coat Protein Gene |
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| Zhuang Mu, Wang Xiaowu, Li Yanshuang,et al..Cloning and Sequence Analysis of Turnip Mosaic Virus Coat Protein Gene[J].China Vegetables,2006,1(3):6-8. |
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| Authors: | Zhuang Mu Wang Xiaowu Li Yanshuang |
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| Institution: | Insititute of Vegetables and Flowers; Chinese Academy of Agricultural Sciences; Beijing 100081; Center of Vocational Education of Jizhou City |
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| Abstract: | Two samples of turnip mosaic virus,which infected cabbage and turnip in Beijing,were named TuMV - BJ1 and TuMV - BJ2, respectively. The CP genes of TuMV - BJ1 and TuMV - BJ2 were sequenced after being cloned into pMD18 -T Vector,and were compared with the CP gene sequences of TuMV reported in GenBank. The results showed that the CP genes of TuMV - BJ1 and TuMV - BJ2 were all 867 nucleotides and coded 288 amino acids in length, and nucleotids homology was 97.2 % and amino acids homology was 97.6 %. They all shared, with the CP gene sequences of TuMV reported in GenBank,nucleotid homologies of 87.8 % - 97.8 % and amino acid homologies of 91.7 % - 99.0 %. Based on the phylogenetic analysis of the CP gene sequences,TuMV - BJ1 and TuMV - BJ2 belonged to world - B group of TuMV. |
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| Keywords: | TuMV Coat protein gene Sequence analysis |
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