| 花生青枯菌潜伏侵染的酶联免疫血清检测技术 |
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| 引用本文： | 单志慧,廖伯寿,谈宇俊,李栋,雷永,沈明珍.花生青枯菌潜伏侵染的酶联免疫血清检测技术[J].中国油料作物学报,1997(2). |
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| 作者姓名： | 单志慧 廖伯寿 谈宇俊 李栋 雷永 沈明珍 |
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| 作者单位： | 中国农科院油料作物研究所!武汉，430062，中国农科院油料作物研究所!武汉，430062，中国农科院油料作物研究所!武汉，430062，中国农科院油料作物研究所!武汉，430062，中国农科院油料作物研究所!武汉，430062，中国农科院油料作物研究所!武汉，430062 |
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| 摘 要： | 采用酶联免疫吸附（ELISA）技术对花生青枯菌（Pseudomonassolanacearum）潜伏侵染进行检测，检测灵敏度为104菌／ml，对潜伏侵染的检测可准确到组织水平，样品制备可用缓冲液浸泡代替研磨，样品保存时间不影响检测效果。
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| 关 键 词： | 青枯菌 潜伏侵染 花生 ELISA |
DETECTION TECHNIQUE FOR LATENT INFECTION OF GROUNDNUT BACTERIAL WILT |
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| Shan Zhihui,Liao Boshou,Tan Yujun,Li Dong,Lei Yong,Shen Mingzhen.DETECTION TECHNIQUE FOR LATENT INFECTION OF GROUNDNUT BACTERIAL WILT[J].Chinese Journal of Oil Crop Sciences,1997(2). |
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| Authors: | Shan Zhihui Liao Boshou Tan Yujun Li Dong Lei Yong Shen Mingzhen |
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| Abstract: | Enzyme-linked immunosobent assay was carried out to detect the latent infection of groundnut bacterial wilt. The sensitivity of this assay was 104/ml. Latent infection could be proved over histological level. Detected sample could be prepared by macerating infected tissue with buffer instead of grinding. The storage duration of sample didn't affect the assay result. |
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| Keywords: | Pseudomonas solanacearum Latent infection Groundnut ELISA |
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