| 高羊茅CBF转录激活因子基因片段的克隆 |
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| 引用本文： | 吴关庭,胡张华,郎春秀,陈笑芸,陈锦清,夏英武.高羊茅CBF转录激活因子基因片段的克隆[J].核农学报,2004,18(5):353-356. |
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| 作者姓名： | 吴关庭 胡张华 郎春秀 陈笑芸 陈锦清 夏英武 |
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| 作者单位： | 浙江大学原子核农业科学研究所,浙江,杭州,310029;浙江省农业科学院病毒学与生物技术研究所,浙江,杭州,310021;浙江省农业科学院病毒学与生物技术研究所,浙江,杭州,310021;浙江大学原子核农业科学研究所,浙江,杭州,310029 |
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| 摘 要： | 根据拟南芥CBF基因序列的保守区设计 1对特异引物 ,采用PCR方法从高羊茅基因组中扩增出 1个DNA片段并克隆到pUCm T载体中。经序列测定和分析 ,该片段长 60 8bp ,与 3个拟南芥CBF基因的核苷酸及其推导氨基酸序列分别具有 81～ 84%和 75～ 79%的同源性 ,而且推导氨基酸序列中包含有同源性更高的AP2DNA结合域以及CBF蛋白的两段特征序列PKK RPAGRxKFxETRHP和DSAWR。这些结果表明 ,本研究克隆的片段为高羊茅CBF基因片段
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| 关 键 词： | 高羊茅 CBF转录激活因子 CBF基因 克隆 |
| 文章编号： | 1000-8551(2004)05-353-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2003年3月14日 |
MOLECULAR CLONING OF A GENE FRAGMENT ENCODING CBF TRANSCRIPTIONAL ACTIVATOR FROM TALL FESCUE |
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| WU Guan-ting , HU Zhang-hua LANG Chun-xiu CHEN Xiao-yun CHEN Jin qing XIA Ying wu.MOLECULAR CLONING OF A GENE FRAGMENT ENCODING CBF TRANSCRIPTIONAL ACTIVATOR FROM TALL FESCUE[J].Acta Agriculturae Nucleatae Sinica,2004,18(5):353-356. |
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| Authors: | WU Guan-ting HU Zhang-hua LANG Chun-xiu CHEN Xiao-yun CHEN Jin qing XIA Ying wu |
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| Institution: | WU Guan-ting 1,2 HU Zhang-hua 2 LANG Chun-xiu 2 CHEN Xiao-yun 2 CHEN Jin qing 2 XIA Ying wu 1 |
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| Abstract: | A DNA fragment was amplified from genome of tall fescue by polymerase chain reaction using a pair of primers designed from the conserved sequences of three Arabidopsis CBF genes, and cloned into the vector pUCm T. Sequence measurement and analysis showed that the nucleotide and deduced amino acid sequences of the cloned 608 bp fragment were 81%～84% and 75%～79% identical to the three Arabidopsis CBF genes respectively. Moreover, there were an AP2 DNA binding domain of higher identity and two signature sequences of CBF proteins, PKK/RPAGRxKFxETRHP and DSAWR, in the deduced amino acid sequence. These results indicated that a tall fescue CBF gene fragment was obtained in this study. |
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