| PEG渗调引发处理对菜薹老化种子DNA损伤的修复作用 |
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| 引用本文： | 乔爱民,傅家瑞.PEG渗调引发处理对菜薹老化种子DNA损伤的修复作用[J].园艺学报,2000,27(1):62-64. |
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| 作者姓名： | 乔爱民 傅家瑞 |
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| 作者单位： | 1. 仲恺农业技术学院园艺系,广州,510225
2. 中山大学生物科学与技术系,广州,510275 |
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| 基金项目： | 广东省自然科学基金!(960 0 68)资助项目，广东省自然科学基金博士项目!(984 2 0 7) |
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| 摘 要： | 利用ＲＡＰＤ技术对菜薹人工老化种子ＤＮＡ的损伤及ＰＥＧ渗调引发处理的修复作用进行了研究。在６１种引物中，有６种在菜薹人工老化的种子上获得了基因组ＤＮＡ指纹图谱，平均每种引物扩增的总ＤＮＡ带数为２．１６，多态性ＤＮＡ带数为１．１７，有１１种引物在ＰＥＧ渗调引发的菜薹老化种子上获得了基因组ＤＮＡ指纹图谱，平均每种引物扩增的总ＤＮＡ带数为３．９１，多态性ＤＮＡ带数也是３．９１。人工老化对菜薹种子基因组Ｄ
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| 关 键 词： | 菜薹 种子 PEG渗调引发 RAPD DNA损伤 修复作用 |
| 修稿时间： | 1999-01-22 |
RAPD Analysis of PEG Osmoprimed Repairs on Damaged DNA in Artificial Aged Seeds of Flowering Chinese Cabbage |
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| Qiao Aimin,Fu Jiarui.RAPD Analysis of PEG Osmoprimed Repairs on Damaged DNA in Artificial Aged Seeds of Flowering Chinese Cabbage[J].Acta Horticulturae Sinica,2000,27(1):62-64. |
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| Authors: | Qiao Aimin Fu Jiarui |
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| Abstract: | In this paper,RAPD was applied to genomic DNA polymorphism analysis of artificial aged and PEG osmoprimed artificial aged seed of flowering chinese cabbage.Among 61 primers used,6 amplified genomic DNA fingerprints on artificial aged seeds,the average number of DNA bands amplified by each primer was 2.16,and number of polymorphic DNA bands was 1.17.11 amplified genomic DNA fingerprints on PEG osmoprimed artificial aged seeds,the average number of DNA bands amplified by each primer was 3.91,and number of polymorphic DNA bands was 3.91. Damage on genomic DNA of flowering Chinese cabbage by artificial accelerated ageing were obvious.With ageing, DNA bands amplified were weaker and weaker till they disappeared completely.Damaged genomic DNA of artificial aged seeds of flowering Chinese cabbage could be repaired through PEG osmopriming.Stronger and/or new DNA bands were amplified from PEG osmoprimed artificial aged seeds. |
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| Keywords: | Flowering chinese cabbage Seed Genomic DNA PEG osmopriming RAPD |
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