| 禾谷镰孢单端孢霉烯族毒素在小麦组织中的积累 |
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| 引用本文： | 俞刚,陈利锋,XIE Wei-ping,柴一秋.禾谷镰孢单端孢霉烯族毒素在小麦组织中的积累[J].植物病理学报,2002,32(2):142-146. |
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| 作者姓名： | 俞刚 陈利锋 XIE Wei-ping 柴一秋 |
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| 作者单位： | 1 南京农业大学植病系农业部病虫监测与治理重点开放实验室, 南京 210095;2 Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, USA |
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| 基金项目： | 国家自然科学基金资助项目 (39870 4 71) |
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| 摘 要： | 本试验将禾谷镰孢一高产毒菌株B4-1采用单小花注滴法接种于小麦抗赤霉病品种苏麦3号和感病品种宁麦6号,用气/质联检法测定接种后4、8、12、16和20 d组织中的单端孢霉烯族毒素积累量。结果显示,在病组织中有脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)、15-乙酰脱氧雪腐镰刀菌烯醇(15-acetyldeoxynivalenol,15-ADON)和3-乙酰脱氧雪腐镰刀菌烯醇(3-acetyldeoxynivalenol,3-ADON)等3种单端孢霉烯族毒素,但未发现雪腐镰刀菌烯醇(nivalenol)和镰刀菌酮-X (fusarenon-X)等毒素。抗病品种病穗组织中,DON平均含量由接种后4 d的29.5 μg/g dry weight (gdw)下降到20d时的15.7μg/gdw,15-ADON和3-ADON量则较低,分别为1.5~3.6μg/gdw和<1.7μg/gdw;而同期感病品种病穗组织中的DON量则由23.1μg/gdw上升到47.7μg/gdw,15-ADON和3-ADON量均较高,分别达8.6~10.0μg/gdw和2.6~8.3μg/gdw。在抗、感病品种无症状穗组织中,3种毒素的含量均很低(<0.5 μg/gdw)。同时,本研究还首次在感病品种的穗颈(总花梗,peduncle)组织中检测到大量的DON和15-ADON及少量的3-ADON。
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| 关 键 词： | 禾谷镰孢 单端孢霉烯族毒素 积累 小麦 穗 穗颈 |
| 文章编号： | 0412-0914(2002)02-0142-05 |
| 修稿时间： | 2001年3月28日 |
ACCUMULATION OF TRICHOTHECENES OF Fusarium graminearum IN WHEAT TISSUES |
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| XIE Wei-ping.ACCUMULATION OF TRICHOTHECENES OF Fusarium graminearum IN WHEAT TISSUES[J].Acta Phytopathologica Sinica,2002,32(2):142-146. |
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| Authors: | XIE Wei-ping |
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| Institution: | 1 Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China;2 Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, USA |
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| Abstract: | The Sumai 3 and Ningmai 6, resistant and susceptible, respectively, to wheat head scab caused by Fusarium graminearum, were inoculated using the single floret inoculation method with B4-1, a F. graminearum strain with high trichothecene production potential. When the spike and peduncle tissues were assayed for trichothecenes after inoculation, deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-ADON) and 3-acetyldeoxynivalenol (3-ADON) were detected, but no nivalenol and fusarenon -X were found. During the period from 4 to 20 days after inoculation, average concentration of DON in symptomatic spikes of Sumai 3 decreased from 29.5 to 15.7 μg/g dry weight (gdw), while that in Ningmai 6 increased from 23.1 to 47.7 μg/gdw. The concentrations of 15-ADON and 3-ADON in symptomatic spikes were higher in Ningmai 6 (8.6-10.0 μg/gdw and 2.6-8.3 μg/gdw, respectively) than those in Sumai 3 (1.5-3.6 μg/gdw and <1.7 μg/gdw, respectively). All three trichothecenes were less than 0.5 μg/gdw in asymptomatic spikes of both Sumai 3 and Ningmai 6. Although no trichothecenes were found in peduncles of Sumai 3, a large amount of DON and 15-ADON (61.7 μg/gdw and 55.3 μg/gdw,respectively) and a small amount of 3-ADON(2.3 μg/gdw) were identified in peduncles of Ningmai 6. This is the first report that trichothecenes are produced in the peduncles of susceptible cultivars. |
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| Keywords: | Fusarium graminearum trichothecenes accumulation wheat spike peduncle |
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