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| IBV安徽分离株膜蛋白基因的克隆及序列分析 | |
| 引用本文： | 毛火云,潘玲,张小飞,廖俊伟,尹秀凤,黄显明,陈申秒.IBV安徽分离株膜蛋白基因的克隆及序列分析[J].畜牧与兽医,2009,41(3). |
| 作者姓名： | 毛火云 潘玲 张小飞 廖俊伟 尹秀凤 黄显明 陈申秒 |
| 作者单位： | 1. 安徽农业大学动物科技学院,安徽合肥230036;南京天邦生物科技有限公司,江苏南京211102 2. 安徽农业大学动物科技学院,安徽合肥,230036 3. 南京天邦生物科技有限公司,江苏南京211102;江苏省农业科学院,江苏南京210014 4. 安徽农业大学动物科技学院,安徽合肥230036;江苏省农业科学院,江苏南京210014 5. 江苏省农业科学院,江苏南京,210014 |
| 摘 要： | 利用设计的1对特异性引物,通过RT-PCR方法扩增出4株鸡传染性支气管炎病毒(IBV)安徽地方分离株膜蛋白M基因全长片段并进行了克隆测序。将各IBV安徽地方分离株与GenBank中注册的一些毒株M基因核苷酸序列及推导的氨基酸序列进行比较和系统进化关系分析,发现毒株间核苷酸序列同源性为88.5%~100%,其相应的氨基酸序列同源性为90.3%~100%;不同毒株间存着重组、缺失、插入及点突变等变异,从ATG至第140 bp区段的核苷酸序列变异频率最高;4株分离毒株属于同一个进化群的2个不同进化亚群,与我国常用疫苗毒株H120、M41和W 93不属同一个进化亚群。 |
| 关 键 词： | 传染性支气管炎病毒 安徽分离株 膜蛋白基因 克隆 序列分析 |
Cloning and sequencing of M genes of infectious bronchitis virus isolated from Anhui Province |
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| MAO Huo-yun,PAN Ling,ZHANG Xiao-fei,LIAO Jun-wei,YIN Xiu-feng,HUANG Xian-ming,CHEN Shen-miao.Cloning and sequencing of M genes of infectious bronchitis virus isolated from Anhui Province[J].Animal Husbandry & Veterinary Medicine,2009,41(3). | |
| Authors: | MAO Huo-yun PAN Ling ZHANG Xiao-fei LIAO Jun-wei YIN Xiu-feng HUANG Xian-ming CHEN Shen-miao |
| Abstract: | A pair of primers were designed and synthesized according to the published matrix(M) protein gene sequences of infectious bronchitis virus(IBV).The full lengths of M genes of 4 strains of IBV isolated from Anhui were amplified by RT-PCR.The amplified products were cloned and sequenced.The nucleotide sequences and the deduced amino acid sequences of M protein genes from the above isolates were compared with the published sequences of reference strains in GenBank,and the phylogenetic tree was constructed.The results showed that there were 88.5% to 100% of nucleotide sequence homology and 90.3% to 100%of deduced amino acid sequence homology among the strains.There were recombinations,deletions,insertions or mutations in the IBV isolates from Anhui.Furthermore,the fragment from start codon ATG to 140 bp showed the most variation;on the basis of the phylogenetic tree of M gene,it appeared that the IBV isolates in Anhui could be divided into two genetic subgroups,and were not in the same subgroup as the vaccine strains H120,M41 and W93 in china. |
| Keywords: | infectious bronchitis virus Anhui M gene cloning sequence analysis |
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