The liver parenchymal cells of rainbow trout (Salmo gairdneri) endocytose mannose-terminated glycoproteins |
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| Authors: | Randi Fritzvold Dr Birgit H Dannevig Trond Berg |
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| Institution: | (1) Department of Biology, Division of General Physiology, University of Oslo, Blindern, P.O. Box 1051, 0316 Oslo 3, Norway;(2) Institute for Nutrition Research, University of Oslo, Blindern, 0316 Oslo 3, Norway |
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| Abstract: | A mannose-terminated glycoprotein,125I-invertase, was taken up and degraded by isolated rainbow trout liver cells at 12°C. The uptake was inhibited by EGTA and no degradation occurred in the presence of ammonium ions. The liver cell suspension was fractionated by differential centrifugation in parenchymal and nonparenchymal cells, respectively. The parenchymal liver cells seemed to be the most active cells in uptake of labelled invertase bothin vitro andin vivo. Only negligible amounts of ligand were recovered in the nonparenchymal cells. Internalization of125I-invertase at different temperatures was demonstrated indirectly by releasing surface-bound ligand with EGTA. Ligand was internalized even at 0°C in trout liver cells.In vitro uptake of125I-invertase was inhibited by excess unlabelled invertase, by mannan and by N-acetylglucosamine.These data suggest that invertase is endocytosed by a mannose-specific pathway by the parenchymal liver cells of rainbow trout. |
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| Keywords: | rainbow trout liver endocytosis mannose receptor |
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