| A/Grganey/SanJiang/160/2006(H5N2)分离株HA基因克隆及序列分析 |
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| 引用本文： | 刘熠楠,李晓冰,柴洪亮,张兰兰,张进,华育平.A/Grganey/SanJiang/160/2006(H5N2)分离株HA基因克隆及序列分析[J].东北林业大学学报,2009,37(7). |
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| 作者姓名： | 刘熠楠 李晓冰 柴洪亮 张兰兰 张进 华育平 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 摘 要： | 应用RT-PCR方法,对从一只野生白眉鸭(Anas querquedula)中分离出的禽流感病毒分离株A/Grganey/SanJiang/160/2006(H5N2)的HA基因进行了序列的克隆和测定.测序结果显示,克隆的HA基因全长为1 731 bp,共编码565个氨基酸.核苷酸和氨基酸的最大同源性比较结果表明,该毒株的HA基因与其它31株H5N2亚型禽流感病毒HA基因的同源性为71.0%～98.2%,氨基酸序列同源性为84.0%～98.2%;遗传进化关系表明,该毒株属于欧亚群系.通过血凝素裂解位点的氨基酸序列分析可知,该毒株的HA切割位点上未见到典型的高致病力毒株H5、H7所具有的一系列碱性氨基酸,符合低致病力禽流感毒株的分子特征.
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| 关 键 词： | H5N2亚型 禽流感病毒 HA基因 序列分析 |
Cloning and Sequence Analysis of HA Gene of A/Garganey/SanJiang/160/2006(H5N2) |
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| Liu Yinan,Li Xiaobing,Chai Hongliang,Zhang Lanlan,Zhang Jin,Hua Yuping.Cloning and Sequence Analysis of HA Gene of A/Garganey/SanJiang/160/2006(H5N2)[J].Journal of Northeast Forestry University,2009,37(7). |
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| Authors: | Liu Yinan Li Xiaobing Chai Hongliang Zhang Lanlan Zhang Jin Hua Yuping |
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| Institution: | Liu Yinan,Li Xiaobing,Chai Hongliang,Zhang Lanlan,Zhang Jin,Hua Yuping(College of Wildlife Resources,Northeast Forestry University,Harbin 150040,P. R. China) |
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| Abstract: | The hemagglutinin (HA) gene of A/Garganey/SanJiang/160/2006(H5N2) isolated from a wild garganey was amplified by RT-PCR and sequenced. The sequencing result showed that the HA complete nucleotide sequence was 1731bp,encoding 565 amino acids. Comparative analysis of isolate A/Garganey/SanJiang/160/2006 with some other isolates of H5N2 subtype indicated that the nucleotide homology ranged from 71.0 percent to 98.2 percent and the amino acid homology was between 84.0 percent and 98.2 percent. Analysis of phylo... |
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| Keywords: | H5N2 Avian influenza virus Hemagglutinin gene Sequence analysis |
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