| 鸡白痢沙门氏菌的分离与鉴定(英文) |
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| 引用本文： | 安辉,张大华,陈小玲,徐福洲,贺云霞,黄秀芬,李桂萍,孙惠玲.鸡白痢沙门氏菌的分离与鉴定(英文)[J].农业科学与技术,2012(3):661-663,668. |
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| 作者姓名： | 安辉 张大华 陈小玲 徐福洲 贺云霞 黄秀芬 李桂萍 孙惠玲 |
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| 作者单位： | 北京市农林科学院畜牧兽医研究所;内蒙古民族大学生命科学学院;山东省潍坊市寒亭区畜牧兽医局 |
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| 基金项目： | Supported by National Natural Science Foundation of China (30871867);Youth Fund Project of Beijing Academy of Agriculture and Forestry Science (QNJJ201012)~~ |
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| 摘 要： | 目的]探索20日龄雏鸡出现有关节肿胀,拉白色稀粪和少量死亡等症状的疾病病因,为鸡场的疾病诊断和防治提供建议和有效措施。方法]采集病鸡样品并进行了细菌学检验。通过细菌分离、PCR、生化试验和回鸡试验鉴定出5株鸡白痢沙门氏菌。结果]用这些分离株感染SPF雏鸡,能复制出与自然感染一致的病例。结论]鸡白痢沙门氏菌是造成该养殖场本次疾病的主要病原。
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| 关 键 词： | 鸡白痢 鸡白痢沙门氏菌 实验室诊断 |
Isolation and Identification of Salmonella pullorum |
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| Hui AN,Dahua ZHANG,Xiaoling CHEN,Fuzhou XU,Yunxia HE,Xiufen HUANG,Guiping LI,Huiling SUN.Isolation and Identification of Salmonella pullorum[J].Agricultural Science ＆ Technology,2012(3):661-663,668. |
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| Authors: | Hui AN Dahua ZHANG Xiaoling CHEN Fuzhou XU Yunxia HE Xiufen HUANG Guiping LI Huiling SUN |
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| Institution: | 1* 1.Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;2.College of Life Science,Inner Mongolia University for the Nationalities,Tongliao 028000,China;3.Hanting District Animal Husbandry and Veterinary Bureau of Weifang,Weifang 261100,China |
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| Abstract: | Objective] The paper was to explore the cause of disease with symptoms of swelling joint,blanc-loose stool and few deaths,so as to provide suggestions and effective measures for diagnosis and control of the disease in chicken farm.Method] The diseased chick samples were collected for bacteriological examination.Through bacterial isolation culture,PCR identification,biochemical test and animal test,5 strains of S.pullorum were identified.Result] When the isolates were inoculated into SPF chicks,the same disease cases with natural infection could be reproduced.Conclusion] S.pullorum is the major cause of the disease in chicken farm. |
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| Keywords: | Pullorum disease Salmonell pullorum Laboratory diagnosis |
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